A lentiviral vaccine expressing KMP11-HASPB fusion protein increases immune response to Leishmania major in BALB/C

Parasitology Research
N MortazavidehkordiS H Hejazi

Abstract

Hydrophilic acylated surface protein B (HASPB) is an immunogenic Leishmania-specific protein that antibodies are produced against it in the sera of Leishmania-infected individuals. Kinetoplastid membrane protein 11 (KMP11) is another Leishmania antigen and considered as the suitable candidate for vaccine development Leishmaniasis. It is a highly conserved surface protein expressed in both promastigotes and amastigotes. In this study, KMP11 and HASPB coding sequences were cloned into a pCDH-cGFP lentiviral vector as a fusion protein to be used as a DNA vaccine against L. major. The KMP11-HASPB fusion protein was successfully expressed as evidenced by RT-PCR and Western blot assays. The effect of the vaccine was determined by evaluating the level of IFN-γ, IL-10, IgG1, and IgG2a performed using ELISA as well as determining the parasite load after challenge with L. major in vaccinated mice. The results revealed that IFN-γ, IL-10, IgG1, and IgG2a significantly increased after vaccination using KMP11-HASPB-expressing lentiviruses in BALB/c mice. It is noteworthy that the level of IFN-γ and IgG2a was higher than that of IL-10 and IgG1, respectively, which indicates the activation Th1 cells, macrophages, and cellular immunity. Moreove...Continue Reading

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Citations

Oct 22, 2019·Human Vaccines & Immunotherapeutics·Beatriz Coutinho De OliveiraValéria Rêgo Alves Pereira
Aug 14, 2020·Parasites & Vectors·Samira SalariPooya Ghasemi Nejad Almani
Feb 21, 2021·Microbial Pathogenesis·Samira SalariPooya Ghasemi Nejad Almani

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Datasets Mentioned

BETA
AJ237587.1

Methods Mentioned

BETA
transfection
flow cytometry
Protein Assay
electrophoresis
PCR
ELISA

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