PMID: 9525620Apr 3, 1998Paper

A leucine zipper-like domain is essential for dimerization and encapsidation of bluetongue virus nucleocapsid protein VP4

Journal of Virology
N RamadeviP Roy

Abstract

The bluetongue virus (BTV) minor protein VP4, with molecular mass of 76 kDa, is one of the seven structural proteins and is located within the inner capsid of the virion. The protein has a putative leucine zipper near the carboxy terminus of the protein. In this study, we have investigated the functional activity of this putative leucine zipper by a number of approaches. The putative leucine zipper region (amino acids [aa] 523 to 551) was expressed initially as a fusion protein by using the pMAL vector of Escherichia coli, which expresses a maltose binding monomeric protein. The expressed fusion protein was purified by affinity chromatography, and its size was determined by gel filtration chromatography. Proteins of two sizes, 51 and 110 kDa, were recovered, one equivalent to the monomeric form and the other equivalent to the dimeric form of the fusion protein. To prove that the VP4-derived sequence was responsible for dimerization of this protein, a mutated fusion protein was created in which a VP4 leucine residue (at aa 537) within the zipper was replaced by a proline residue. Analyses of the mutated protein demonstrated that the single mutation indeed prevented dimerisation of the protein. The dimeric nature of VP4 was furth...Continue Reading

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Citations

Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·N RamadeviP Roy
Sep 10, 2014·Viruses·Bjorn-Patrick Mohl, Polly Roy
May 10, 2005·Journal of Cellular Physiology·Arianna Loregian, Giorgio Palù
Feb 10, 2006·Cell Research·Chang Zhen LiuSen Fang Sui
Apr 10, 2007·Nature Structural & Molecular Biology·Geoff SuttonPolly Roy
Jul 18, 2008·The Journal of General Virology·Polly Roy
Jun 19, 2016·Nucleic Acids Research·Anand RamanathanSiu-Hong Chan
Dec 26, 2002·Journal of Molecular Recognition : JMR·A V VeselovskyP Janssen
Jul 16, 2004·Journal of Virology·Emma L NasonPolly Roy
Sep 19, 2002·Biochemical and Biophysical Research Communications·Hideki YamaguchiTadaomi Takenawa

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