Abstract
The bluetongue virus (BTV) minor protein VP4, with molecular mass of 76 kDa, is one of the seven structural proteins and is located within the inner capsid of the virion. The protein has a putative leucine zipper near the carboxy terminus of the protein. In this study, we have investigated the functional activity of this putative leucine zipper by a number of approaches. The putative leucine zipper region (amino acids [aa] 523 to 551) was expressed initially as a fusion protein by using the pMAL vector of Escherichia coli, which expresses a maltose binding monomeric protein. The expressed fusion protein was purified by affinity chromatography, and its size was determined by gel filtration chromatography. Proteins of two sizes, 51 and 110 kDa, were recovered, one equivalent to the monomeric form and the other equivalent to the dimeric form of the fusion protein. To prove that the VP4-derived sequence was responsible for dimerization of this protein, a mutated fusion protein was created in which a VP4 leucine residue (at aa 537) within the zipper was replaced by a proline residue. Analyses of the mutated protein demonstrated that the single mutation indeed prevented dimerisation of the protein. The dimeric nature of VP4 was furth...Continue Reading
References
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 3, 1978·Biochemistry·M Levitt
Aug 1, 1992·Virology·H Le BloisP Roy
Oct 25, 1991·Science·E K O'SheaT Alber
Dec 7, 1990·Science·J C HuR T Sauer
Apr 1, 1990·Journal of Virology·T J French, P Roy
Oct 11, 1990·Nucleic Acids Research·P A KittsR D Possee
Jun 1, 1990·AIDS Research and Human Retroviruses·E L DelwartT Gilmore
Jun 1, 1989·Genes & Development·L J RansoneI M Verma
Feb 16, 1989·Nature·C V DangW M Lee
Sep 11, 1987·Nucleic Acids Research·Y D YuP Roy
Jun 24, 1988·Science·W H LandschulzS L McKnight
Jun 5, 1988·Journal of Molecular Biology·D J Barlow, J M Thornton
Jan 15, 1974·Biochemistry·P Y Chou, G D Fasman
Aug 15, 1970·Nature·U K Laemmli
May 1, 1995·Journal of Virology·H B BernsteinE Hunter
Aug 1, 1993·Virology·I J HuangJ K Li
May 1, 1996·Journal of Virology·D C ShugarsT J Matthews
Jun 1, 1996·Virology·E E RamsdaleA J Kingsman
Citations
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·N RamadeviP Roy
Sep 10, 2014·Viruses·Bjorn-Patrick Mohl, Polly Roy
May 10, 2005·Journal of Cellular Physiology·Arianna Loregian, Giorgio Palù
Feb 10, 2006·Cell Research·Chang Zhen LiuSen Fang Sui
Apr 10, 2007·Nature Structural & Molecular Biology·Geoff SuttonPolly Roy
Jul 18, 2008·The Journal of General Virology·Polly Roy
Jun 19, 2016·Nucleic Acids Research·Anand RamanathanSiu-Hong Chan
Dec 26, 2002·Journal of Molecular Recognition : JMR·A V VeselovskyP Janssen
Dec 15, 2005·Journal of Virology·Christoph WirblichPolly Roy
Jul 16, 2004·Journal of Virology·Emma L NasonPolly Roy
May 16, 2012·Virus Genes·Min-Juan ZhangChuan-Xi Zhang
Feb 7, 2021·Viruses·Paula Lagan TregaskisVictoria J Smyth
Sep 19, 2002·Biochemical and Biophysical Research Communications·Hideki YamaguchiTadaomi Takenawa