A linear megaplasmid, p1CP, carrying the genes for chlorocatechol catabolism of Rhodococcus opacus 1CP

Microbiology
Christina KönigMichael Schlömann

Abstract

The Gram-positive actinobacterium Rhodococcus opacus 1CP is able to utilize several (chloro)aromatic compounds as sole carbon sources, and gene clusters for various catabolic enzymes and pathways have previously been identified. Pulsed-field gel electrophoresis indicates the occurrence of a 740 kb megaplasmid, designated p1CP. Linear topology and the presence of covalently bound proteins were shown by the unchanged electrophoretic mobility after S1 nuclease treatment and by the immobility of the native plasmid during non-denaturing agarose gel electrophoresis, respectively. Sequence comparisons of both termini revealed a perfect 13 bp terminal inverted repeat (TIR) as part of an imperfect 583/587 bp TIR, as well as two copies of the highly conserved centre (GCTXCGC) of a palindromic motif. An initial restriction analysis of p1CP was performed. By means of PCR and hybridization techniques, p1CP was screened for several genes encoding enzymes of (chloro)aromatic degradation. A single maleylacetate reductase gene macA, the clc gene cluster for 4-chloro-/3,5-dichlorocatechol degradation, and the clc2 gene cluster for 3-chlorocatechol degradation were found on p1CP whereas the cat and pca gene clusters for the catechol and the proto...Continue Reading

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Citations

Sep 1, 2005·Applied Microbiology and Biotechnology·Susanne NiescherMichael Schlömann
Apr 25, 2012·Applied Biochemistry and Biotechnology·Dirk TischlerMichael Schlömann
Jun 18, 2005·Current Opinion in Biotechnology·Michael J LarkinChristopher C R Allen
Jun 2, 2009·Journal of Bacteriology·Dirk TischlerMichael Schlömann
Oct 24, 2009·Plasmid·Julian R DibFriedhelm Meinhardt
Feb 26, 2005·Journal of Anatomy·R C PayneA M Wilson
Dec 22, 2004·Journal of Anatomy·R C PayneA M Wilson
Jan 21, 2006·Environmental Microbiology·Mitsuo SekineShigeaki Harayama
Mar 1, 2009·Microbial Biotechnology·Laura Tomás-GallardoBelén Floriano
May 7, 2011·Equine Veterinary Journal·S N PassmanJ J Rawlinson
Dec 12, 2013·Microbial Biotechnology·Laura Tomás-GallardoBelén Floriano
Jun 18, 2005·Journal of Anatomy·R C PayneA M Wilson
Oct 7, 2014·FEMS Microbiology Letters·Janosch A D GröningMichael Schlömann
Oct 19, 2014·Applied and Environmental Microbiology·Francesca BottaciniDouwe van Sinderen
Aug 9, 2007·International Journal of Systematic and Evolutionary Microbiology·Rolf-Michael WittichWolf-Rainer Abraham
May 2, 2019·Frontiers in Microbiology·Dirk TischlerMarco W Fraaije

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