PMID: 9426199Jan 13, 1998Paper

A linear region of a monoclonal antibody conformational epitope mapped on p185HER2 oncoprotein

Biological Chemistry
R OrlandiM Colnaghi

Abstract

Analysis of epitopes recognized by therapeutic monoclonal antibodies (mAb) is critical in clinical applications and in structure/function studies of target antigen. mAb MGr6 recognizes the extracellular domain of the p185HER2 oncoprotein and is a promising candidate for cancer immunodiagnosis and immunotherapy. Thus, epitope location and structure on p185HER2 need to be investigated. The use of MGr6-selected phage-displayed peptides for epitope analysis served to dissect the MGr6 epitope into at least two subregions, mimicked by CHSDC- and (L)P-(L)K(L) phage displayed peptides, respectively. Comparison of amino acid sequences of CHSDC peptides with the p185HER2 protein sequence and analysis of MGr6 reactivity with p185HER2 deletion mutants identified the linear subregion CCHEQCAAG of the MGr6 epitope, corresponding to amino acids 235-243 of the p185HER2 protein. This continuous subregion is part of a larger conformational epitope, and other amino acids, including a proline, a lysine and leucine residues contained in (L)P-(L)K(L) phage-displayed peptides appear to contribute to the formation of the MGr6 epitope surface.

References

Aug 1, 1978·Immunochemistry·M Z Atassi, J A Smith
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P CarterH M Shepard
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J K BatraC R King
Oct 1, 1991·Current Opinion in Biotechnology·G P Smith
Jun 1, 1992·Hybridoma·F CentisM I Colnaghi
Jul 20, 1993·Journal of Molecular Biology·B Rost, C Sander

❮ Previous
Next ❯

Citations

Jul 6, 2000·Immunology Letters·K G GazarianJ P Laclette
Oct 24, 2003·Protein Science : a Publication of the Protein Society·Brian W BaileyEdward A Dratz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.