A link between ferritin gene expression and ribonucleotide reductase R2 protein, as demonstrated by retroviral vector mediated stable expression of R2 cDNA

FEBS Letters
H FanJ A Wright

Abstract

We have constructed a retroviral expression vector for the mammalian ribonucleotide reductase R2 component. Stable infectants, which express a myc epitope tagged R2 protein from the vector cDNA were obtained and described for the first time. Cells containing the recombinant protein exhibited increased ribonucleotide reductase activity, and were resistant to the antitumour agent hydroxyurea, which targets the R2 component of ribonucleotide reductase. Furthermore, a direct link between ferritin gene expression and R2 protein was observed, since cells containing vector expressed recombinant R2 protein exhibited increased H-chain and L-chain ferritin gene expression.

References

Dec 1, 1990·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J A WrightA Y Tagger
Jan 1, 1988·Progress in Nucleic Acid Research and Molecular Biology·J W Drysdale
Jan 1, 1987·Cytogenetics and Cell Genetics·P N ToninW H Lewis
Jan 1, 1993·Methods in Enzymology·A D MillerC M Lynch

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Citations

Jul 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G CairoA Pietrangelo
Aug 19, 2007·Chemical Communications : Chem Comm·Zhen YangZijian Guo
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·H FanJ A Wright
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·H FanJ A Wright
Jan 24, 1998·Critical Reviews in Clinical Laboratory Sciences·H A Goldenberg
Feb 13, 2001·Microscopy Research and Technique·J RussoI H Russo
Aug 16, 2003·The Journal of Biological Chemistry·Malgorzata GoralskaM Christine McGahan
Aug 30, 2000·Journal of the National Cancer Institute. Monographs·J RussoI H Russo

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