A Lipid-Accumulating Alga Maintains Growth in Outdoor, Alkaliphilic Raceway Pond with Mixed Microbial Communities

Frontiers in Microbiology
Tisza A S BellBrent M Peyton

Abstract

Algal biofuels and valuable co-products are being produced in both open and closed cultivation systems. Growing algae in open pond systems may be a more economical alternative, but this approach allows environmental microorganisms to colonize the pond and potentially infect or outcompete the algal "crop." In this study, we monitored the microbial community of an outdoor, open raceway pond inoculated with a high lipid-producing alkaliphilic alga, Chlorella vulgaris BA050. The strain C. vulgaris BA050 was previously isolated from Soap Lake, Washington, a system characterized by a high pH (∼9.8). An outdoor raceway pond (200 L) was inoculated with C. vulgaris and monitored for 10 days and then the culture was transferred to a 2,000 L raceway pond and cultivated for an additional 6 days. Community DNA samples were collected over the 16-day period in conjunction with water chemistry analyses and cell counts. Universal primers for the SSU rRNA gene sequences for Eukarya, Bacteria, and Archaea were used for barcoded pyrosequence determination. The environmental parameters that most closely correlated with C. vulgaris abundance were pH and phosphate. Community analyses indicated that the pond system remained dominated by the Chlorella ...Continue Reading

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Citations

Sep 1, 2018·Journal of Applied Microbiology·S YaoA Schramm
May 3, 2019·BioTechniques·Juliette OhanShawn R Starkenburg
Apr 4, 2021·Plants·Elvira E ZiganshinaAyrat M Ziganshin

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Methods Mentioned

BETA
optical microscopy
454 sequencing
PCR

Software Mentioned

PyNAST
R vegan package
QIIME
CCA
UCLUST
Chimera Slayer
UniFrac
SparCC
Quantitative Insights into Microbial Ecology ( QIIME )

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