A liquid biopsy for bronchopulmonary/lung carcinoid diagnosis

Oncotarget
Mark KiddKyung-Min Chung

Abstract

No effective blood biomarker exists to detect and clinically manage bronchopulmonary (BP) neuroendocrine tumors (NET). We developed a blood-based 51 NET-specific transcript set for diagnosis and monitoring and evaluated clinical performance metrics. It accurately diagnosed the tumor and differentiated stable from progressive disease as determined by RECIST criteria. Gene expression was evaluated in: a) publicly available BPNET transcriptomes (GSE35679); b) two BPNET cell-lines; and c) BPNET tissue with paired blood (n= 7). Blood gene expression was assessed in 194 samples including controls, benign lung diseases, malignant lung diseases and small bowel NETs. A separate validation study in 25 age- and gender-matched BPNETs/controls was performed. Gene expression measured by real-time PCR was scored (0-100%; normal: < 14%). Regression analyses, Principal Component Analysis (PCA), hierarchical clustering, Fisher's and non-parametric evaluations were undertaken. All 51 genes were identified in BPNET transcriptomes, tumor samples and cell-lines. Significant correlations were evident between paired tumor and blood (R2:0.63-0.91,p< 0.001). PCA and hierarchical clustering identified blood gene expression was significantly different bet...Continue Reading

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Citations

Feb 13, 2020·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Pier Luigi FilossoLisa Bodei
Jun 17, 2020·Frontiers in Oncology·Martine BocchiniMassimiliano Mazza
Dec 16, 2019·European Journal of Nuclear Medicine and Molecular Imaging·Lisa BodeiIrvin M Modlin
Jan 22, 2020·Annals of Oncology : Official Journal of the European Society for Medical Oncology·K ÖbergS Paulson
Dec 17, 2019·Advances in Medical Sciences·Anna MalczewskaIrvin M Modlin

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Methods Mentioned

BETA
biopsy
nucleotide exchange
PCR
PCA
blood draw

Software Mentioned

Prism
NETs
Morpheus
TAC
GraphPad
GEP
MedCalc

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