A liquid chromatography-electrospray tandem mass spectrometry method for the determination of antiarrhythmic drugs and their metabolites in forensic whole blood samples

Journal of Analytical Toxicology
Lambert K Sørensen

Abstract

A liquid chromatography-tandem mass spectrometry method, using pneumatically assisted electrospray ionization was developed for the determination of amiodarone, desethylamiodarone, propafenone, N-depropylpropafenone, 5-OH-propafenone, flecainide, and sotalol in human antemortem and postmortem whole blood. A mixture of methanol and acetonitrile was used to extract the samples, and the clear extracts obtained from the protein precipitation were injected directly onto an ethyl-linked phenyl LC column. The isotope dilution technique was applied for quantitative analysis. The relative intralaboratory reproducibility standard deviations were 5-9% at a concentration range of 1-5 mg/L and 9-12% at a concentration of 0.1 mg/L. The mean true recoveries were greater than 91% in the concentration range of 0.05-5 mg/L. The detection limits were in the range of 8-18 µg/L.

References

Nov 15, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·T Breindahl
Feb 5, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Manfred Kollroser, Caroline Schober
Nov 23, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shuijun LiJianying Ren

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Citations

May 6, 2014·Forensic Science International : Synergy·Matthew Di RagoOlaf H Drummer
Jan 15, 2015·Biomedical Chromatography : BMC·Zhiping FengXianhua Zhang
Sep 15, 2012·International Journal of Speech-language Pathology·Man Ling IpSteve An Xue
Aug 29, 2020·American Journal of Speech-language Pathology·Kenneth O St Louis

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