A Liquid Chromatography with Tandem Mass Spectrometry-Based Proteomic Analysis of Primary Cultured Cells and Subcultured Cells Using Mouse Adipose-Derived Mesenchymal Stem Cells

Stem Cells International
Yoshiki NakashimaHirofumi Noguchi

Abstract

Adipose-derived mesenchymal stem cells (MSC-ATs) are representative cell sources for cell therapy. However, how cell stress resulting from passage influences the MSC-AT protein expression has been unclear. In this study, a protein expression analysis was performed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) using mouse primary cultured cells (P0) and cells passaged three times (P3) as samples. A total of 256 proteins were classified as cellular process-related proteins, while 179 were classified as metabolic process-related proteins in P0. These were considered to be adaptive responses of the cells to an in vitro environment. However, seven proteins of growth were identified (Csf1, App, Adam15, Alcam, Tbl1xr1, Ninj1, and Sbds) in P0. In addition, four proteins of antioxidant activity were also identified (Srxn1, Txndc17, Fam213b, and Apoe) in P0. We identified 1139 proteins expressed in both P0 and P3 cells that had their expression decreased to 69.4% in P3 cells compared with P0 cells, but 1139 proteins are very likely proteins that are derived from MSC-AT. The function of MSC-ATs was maintained after three passages. However, the LC-MS/MS analysis data showed that the protein expression was degraded after...Continue Reading

References

Feb 11, 2004·Nature Medicine·Hirofumi NoguchiHideki Matsui
Oct 20, 2007·Proceedings·Shinichi MatsumotoMarlon Levy
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
Jan 12, 2010·Developmental Cell·Sally L Dunwoodie
Nov 5, 2010·Seminars in Thrombosis and Hemostasis·Kristina M LittleKlaus Ley
Sep 8, 2011·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Fariborz MoazamiSafoura Sahebi
Jul 17, 2012·Cell Transplantation·Masayuki ShimodaShinichi Matsumoto
Aug 21, 2014·Methods in Molecular Biology·Hamid-Reza AghayanBabak Arjmand
Mar 24, 2015·Bioscience Reports·Imran UllahGyu Jin Rho
Nov 13, 2015·Hormone Molecular Biology and Clinical Investigation·Amira AlkharusiLeandro Fernandez-Pérez
Feb 3, 2016·Journal of Chromatography. a·Gabriella PocsfalviKároly Vékey
Feb 10, 2016·Cell Medicine·Chika Miyagi-ShiohiraHirofumi Noguchi
Nov 1, 2016·Archivum Immunologiae Et Therapiae Experimentalis·Anna BajekTomasz Drewa
Jul 19, 2016·International Journal of Stem Cells·Diana LegzdinaUldis Berzins
Oct 11, 2016·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Laura FreseSimon P Hoerstrup
Oct 21, 2016·Journal of Cutaneous and Aesthetic Surgery·Shaun S TanWarren Rozen
Feb 12, 2017·Stem Cells Translational Medicine·Jordan A DykstraDaniel J Kota
Jun 10, 2017·Scientific Reports·Hirofumi NoguchiYasufumi Noguchi
Apr 4, 2018·Polish Journal of Veterinary Sciences·S PanasophonkulA Sathanawongs

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

Scaffold
Mascot

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