A live bioprobe for studying diatom-surface interactions

Biophysical Journal
Fernando Terán ArceBarbara Wigglesworth-Cooksey

Abstract

Atomic force microscopy has been employed to compare the adhesion of Navicula species I diatoms to surfaces of a hydrophobic elastomer, Intersleek, and a hydrophilic mineral, mica. This was accomplished using tipless atomic force microscopy cantilevers functionalized with live diatom cells. Both surfaces were tested with the same diatom bioprobe. Force versus distance curves generated during these experiments revealed comparable cell adhesion strengths on Intersleek and mica, indicating that Navicula diatoms secrete extracellular polymeric substances with hydrophobic and hydrophilic properties. A statistical analysis of force curves was carried out and the average values of works of detachment of a diatom from Intersleek and mica surfaces were determined.

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Citations

May 7, 2013·Protoplasma·Jiadao WangDarong Chen
Apr 5, 2005·Biofouling·R HollandMaureen E Callow
Feb 17, 2009·Journal of Bacteriology·Fernando Terán ArceRecep Avci
Oct 12, 2013·International Journal of Molecular Sciences·Vesna SvetličićTea Mišić Radić
Apr 20, 2011·Journal of Molecular Recognition : JMR·Galja PletikapićVera Zutić
Dec 14, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Huabin WangMichelle L Gee
May 15, 2007·Environmental Toxicology·B Wigglesworth-CookseyRichard Long
Jun 14, 2006·The Journal of Biological Chemistry·Fei LiuRatnesh Lal
Jul 20, 2018·Biointerphases·Kim Alexander NolteAxel Rosenhahn
Sep 3, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Beng Joo Reginald ThioArturo A Keller
Nov 27, 2019·ACS Applied Materials & Interfaces·Martin LavialeSofiane El-Kirat-Chatel

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