A local lymph node assay to analyse immunosuppressive effects of topically applied drugs

European Journal of Pharmacology
B HomeyP Lehmann

Abstract

Topical glucocorticosteroids represent the mainstay of antiinflammatory therapy in the treatment of inflammatory skin diseases. Their clinical use, however, is limited by local and systemic side-effects. Thus, in dermatopharmacology there is a large demand for alternative non-steroidal antiinflammatories. Other than transplantation models, most of the frequently used in vivo test systems for assessment of drug-induced immunosuppression measure changes in inflammatory skin responses by means of skin erythema and edema after challenge of sensitized animals. The aim of this study was to develop an alternative mouse model to detect and analyse immunosuppressive effects of topically applied drugs. On the basis of a modified local lymph node assay, we analysed effects of topical hydrocortisone, dexamethasone, mometasone furoate and FK506 (tacrolimus) during the induction phase of contact hypersensitivity. On 4 consecutive days, NMRI mice were treated on the dorsal surfaces of both ears with increasing concentrations of test compound. During the last 3 days, the mice received in addition the contact sensitizer, oxazolone (1%). On day 5, draining auricular lymph nodes were removed in order to assess lymph node cell counts and perform f...Continue Reading

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Citations

Jul 6, 2000·Immunopharmacology·T AssmannT Ruzicka
Mar 5, 1998·Toxicology Letters·H C SchuppeP Kind
May 31, 2011·Toxicology Letters·Keiyu OshidaYohei Miyamoto
May 12, 2009·Ultrasound in Medicine & Biology·Mattéo R BosisioS Lori Bridal
Jan 5, 2002·Seminars in Cutaneous Medicine and Surgery·M R Ling
Jan 22, 2000·Journal of the American Academy of Dermatology·D Richter-HintzT Ruzicka
Mar 31, 1998·Lancet·H C SchuppeT Ruzicka
May 4, 2002·Allergologia et immunopathologia·F J Ortiz de Frutos

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