A Locked Nucleic Acid Probe Based on Selective Salt-Induced Effect Detects Single Nucleotide Polymorphisms

BioMed Research International
Jing ZhangMinjie Wei

Abstract

Detection of single based genetic mutation by using oligonucleotide probes is one of the common methods of detecting single nucleotide polymorphisms at known loci. In this paper, we demonstrated a hybridization system which included a buffer solution that produced selective salt-induced effect and a locked nucleic acid modified 12 nt oligonucleotide probe. The hybridization system is suitable for hybridization under room temperature. By using magnetic nanoparticles as carriers for PCR products, the SNPs (MDR1 C3435T/A) from 45 volunteers were analyzed, and the results were consistent with the results from pyrophosphoric acid sequencing. The method presented in this paper differs from the traditional method of using molecular beacons to detect SNPs in that it is suitable for research institutions lacking real-time quantitative PCR detecting systems, to detect PCR products at room temperature.

References

Nov 12, 2002·Clinical Pharmacology and Therapeutics·Andreas JohneIvar Roots
Jan 17, 2003·Expert Review of Molecular Diagnostics·Peter MouritzenSøren Møller
Feb 8, 2003·Trends in Biotechnology·Michael Petersen, Jesper Wengel
Jul 27, 2005·British Journal of Clinical Pharmacology·Balram ChowbayEdmund J D Lee
Aug 30, 2005·Annual Review of Genomics and Human Genetics·Dana C CrawfordDeborah A Nickerson
Nov 10, 2005·Journal of the American Chemical Society·Lin WangWeihong Tan
Nov 16, 2007·The New England Journal of Medicine·Derek J JonkerMalcolm J Moore
Dec 10, 2008·Angewandte Chemie·Kemin WangWeihong Tan
May 30, 2009·Human Mutation·Carl T Wittwer
Feb 10, 2010·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Tamra E MeyerAaron R Folsom
Jul 5, 2011·Methods in Cell Biology·Maurizio CarbonariMassimo Fiorilli
Nov 17, 2011·Analytical and Bioanalytical Chemistry·Jia GuoNicholas J Turro
Dec 23, 2011·Biosensors & Bioelectronics·Zongrui ZhanSang Jun Sim
Jun 20, 2013·Theranostics·Qi WangJunchen Wu
Aug 12, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Sourav MishraRupa Mukhopadhyay
Feb 26, 2015·Pharmacological Reports : PR·Ewa Jaźwińska-TarnawskaAnna Wiela-Hojeńska

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Fluorescence
electrophoresis
PCR.

Software Mentioned

BLAST

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.