A locus on barley chromosome 5H affects adult plant resistance to powdery mildew

Molecular Breeding : New Strategies in Plant Improvement
Sanjiv GuptaDiane E Mather

Abstract

Adult plant resistance against plant pathogens is of interest as a means to achieve durable resistance. Prior to this research, the barley lines CLE210 (from Uruguay) and Denar (from the Czech Republic) had been reported to exhibit adult-plant resistance against powdery mildew. Here, populations of doubled haploid lines from crosses of these lines with the susceptible cultivar Baudin were evaluated for powdery mildew resistance in field experiments. Using linkage maps constructed from genotyping-by-sequencing (GBS) data, it was determined that differences in resistance were largely attributable to a region on the long arm of chromosome 5H (5HL). Therefore, KASP™ assays were developed based on GBS tag sequences mapped on that chromosome, providing more reliable genetic maps. In each population, a large-effect QTL was mapped on 5HL. As no sequence variation was detected between CLE210 and Denar in this region of 5HL, the two sources of resistance may be identical by descent in the QTL region and carry the same resistance gene. Marker assays from the QTL region were evaluated on a panel of barley lines, providing information that breeders could use to select assays for use in marker-assisted selection.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
May 17, 2011·PloS One·Robert J ElshireSharon E Mitchell
Aug 1, 1994·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M A MaroofQ Zhang
Oct 1, 1991·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·P M RogowskyR M Koebner
May 13, 2014·Methods in Molecular Biology·Sue BroughtonPhilip A Davies

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Citations

May 15, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yonggang WangChengdao Li
Mar 5, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hoan X DinhMohammad Pourkheirandish

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Methods Mentioned

BETA
Genome Sequencing
genotyping

Software Mentioned

R Statistical Computing Environment ( R Core Team
ASMap package
ASReml
GBS
silicoDArT
R package
wgaim
R
ASMap
silicoDArTs

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