A Long-Chain Secondary Alcohol Dehydrogenase from Rhodococcus erythropolis ATCC 4277.

Applied and Environmental Microbiology
B LudwigK Kendall

Abstract

A NAD-dependent secondary alcohol dehydrogenase has been purified from the alkane-degrading bacterium, Rhodococcus erythropolis ATCC 4277. The enzyme was found to be active against a broad range of substrates, particularly long-chain secondary aliphatic alcohols. Although optimal activity was observed with linear 2-alcohols containing between 6 and 11 carbon atoms, secondary alcohols as long as 2-tetradecanol were oxidized at 25% of the rate seen with mid-range alcohols. The purified enzyme was specific for the S-(+) stereoisomer of 2-octanol and had a specific activity for 2-octanol of over 200 (mu)mol/min/mg of protein at pH 9 and 37(deg)C, 25-fold higher than that of any previously reported S-(+) secondary alcohol dehydrogenase. Linear primary alcohols containing between 3 and 13 carbon atoms were utilized 20- to 40-fold less efficiently than the corresponding secondary alcohols. The apparent K(infm) value for NAD(sup+) with 2-octanol as the substrate was 260 (mu)M, whereas the apparent K(infm) values for the 2-alcohols ranged from over 5 mM for 2-pentanol to less than 2 (mu)M for 2-tetradecanol. The enzyme showed moderate thermostability (half-life of 4 h at 60(deg)C) and could potentially be useful for the synthesis of opt...Continue Reading

References

Oct 1, 1991·Applied Microbiology and Biotechnology·D Bernhardt, H Diekmann
Jun 1, 1992·Applied and Environmental Microbiology·E GrundR Eichenlaub
Dec 1, 1988·Applied and Environmental Microbiology·M M HäggblomM S Salkinoja-Salonen
Jul 13, 1972·Biochimica Et Biophysica Acta·J P Tassin, J P Vandecasteele
Aug 1, 1970·Journal of Bacteriology·J E Allen, A J Markovetz
Dec 16, 1970·Biochimica Et Biophysica Acta·J M LebeaultE Azoulay
Feb 1, 1988·Applied and Environmental Microbiology·F O BryantL G Ljungdahl

❮ Previous
Next ❯

Citations

Dec 17, 2008·Applied Biochemistry and Biotechnology·Qilei ChenBaoquan Zhu
May 11, 2011·Biotechnology and Bioengineering·Karthikeyan D RamachandriyaRaymond L Huhnke
May 23, 2014·Applied Microbiology and Biotechnology·Enriqueta Martinez-RojasLeif-Alexander Garbe
May 27, 2014·Environmental Science and Pollution Research International·Hai-Yan YangLi Li
Mar 10, 2001·Environmental Microbiology·V AndreoniL Cavalca
Nov 3, 2020·The Science of the Total Environment·Zhansaya AssilAndrew Free
Aug 16, 2018·Frontiers in Microbiology·Isabelle Raymond-BouchardLyle G Whyte
Jul 2, 1998·Applied and Environmental Microbiology·L G WhyteC W Greer
Jun 24, 2004·Environmental Science & Technology·Mary Joyce A DinglasanScott A Mabury

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.