A long-lived M-like state of phoborhodopsin that mimics the active state

Biophysical Journal
Yuki SudoNaoki Kamo

Abstract

Pharaonis phoborhodopsin (ppR, also called pharaonis sensory rhodopsin II) is a seven transmembrane helical retinal protein. ppR forms a signaling complex with pharaonis Halobacterial transducer II (pHtrII) in the membrane that transmits a light signal to the sensory system in the cytoplasm. The M-state during the photocycle of ppR (lambda(max) = 386 nm) is one of the active (signaling) intermediates. However, progress in characterizing the M-state at physiological temperature has been slow because its lifetime is very short (decay half-time is approximately 1 s). In this study, we identify a highly stable photoproduct that can be trapped at room temperature in buffer solution containing n-octyl-beta-d-glucoside, with a decay half-time and an absorption maximum of approximately 2 h and 386 nm, respectively. HPLC analysis revealed that this stable photoproduct contains 13-cis-retinal as a chromophore. Previously, we reported that water-soluble hydroxylamine reacts selectively with the M-state, and we found that this stable photoproduct also reacts selectively with that reagent. These results suggest that the physical properties of the stable photoproduct (named the M-like state) are very similar with the M-state during the photo...Continue Reading

References

Oct 14, 2000·Annual Review of Cell and Developmental Biology·John L SpudichElena N Spudich
Aug 16, 2001·Proceedings of the National Academy of Sciences of the United States of America·A RoyantJ Navarro
Aug 22, 2001·Biochimica Et Biophysica Acta·Masayuki IwamotoNaoki Kamo
Feb 6, 2002·Biochemistry·Jun SasakiFumio Tokunaga
Aug 9, 2002·Journal of Photochemistry and Photobiology. B, Biology·Yuki SudoNaoki Kamo
Dec 24, 2002·Biophysical Journal·Yuji FurutaniHideki Kandori
Jul 16, 2003·Journal of Molecular Biology·Silke Hippler-MreyenMartin Engelhard
Jun 2, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Johann P KlareHeinz-Jürgen Steinhoff
Jun 14, 2005·The Journal of Biological Chemistry·Vladislav B BergoJohn L Spudich
Sep 15, 2005·The Journal of Biological Chemistry·Enrica BordignonHeinz-Jürgen Steinhoff
Feb 3, 2006·Nature·Rouslan MoukhametzianovValentin I Gordeliy

Related Concepts

SopII protein, Halobacterium salinarium
Dose-Response Relationship, Radiation
Gray Units
Halorhodopsins
Sensory Rhodopsins
High Pressure Liquid Chromatography Procedure
Cytoplasm
Hydroxylamine
Macrophage
Retina

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