A Long Non-coding RNA IVRPIE Promotes Host Antiviral Immune Responses Through Regulating Interferon β1 and ISG Expression

Frontiers in Microbiology
Lingna ZhaoXiliang Wang

Abstract

Accumulating studies have shown that long non-coding RNAs (lncRNAs) modulate multiple biological processes, including immune response. However, the underlying mechanisms of lncRNAs regulating host antiviral immune response are not well elucidated. In this study, we report that analysis of the existing dataset transcriptome of blood immune cells of patients with influenza A virus (IAV) infection and after recovery (GSE108807) identified a novel lncRNA, termed as IVRPIE (Inhibiting IAV Replication by Promoting IFN and ISGs Expression), was involved in antiviral innate immunity. In vitro studies showed that IVRPIE was significantly upregulated in A549 cells after IAV infection. Gain-and-loss of function experiments displayed that enforced IVRPIE expression significantly inhibited IAV replication in A549 cells. Conversely, silencing IVRPIE promoted IAV replication. Furthermore, IVRPIE positively regulates the transcription of interferon β1 and several critical interferon-stimulated genes (ISGs), including IRF1, IFIT1, IFIT3, Mx1, ISG15, and IFI44L, by affecting histone modification of these genes. In addition, hnRNP U was identified as an interaction partner for IVRPIE. Taken together, our findings suggested that a novel lncRNA IVR...Continue Reading

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Citations

Jun 17, 2020·Emerging Microbes & Infections·Jing Wang, Shan Cen
Sep 10, 2020·International Journal of Molecular Sciences·Beatriz SuarezPuri Fortes
Nov 4, 2020·RNA Biology·Katharina Walther, Leon N Schulte
Jan 7, 2021·RNA Biology·Gayan BamunuarachchiLin Liu
Mar 28, 2021·Virus Research·Ashish Prasad, Manoj Prasad
Jul 13, 2021·Reviews in Medical Virology·Mina Mobini KeshehSomayeh Shokri
Aug 19, 2021·Immunological Reviews·Nandan S GokhaleRam Savan

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Datasets Mentioned

BETA
GSE108807

Methods Mentioned

BETA
Antisense oligonucleotides
PCR
Assay
Pull-Down
Immunoprecipitation
RIP
ChIP
transfection
ELISA
pull down

Software Mentioned

IVRPIE
GraphPad
GraphPad Prism

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