A long non-coding RNA regulates cadherin transcription and susceptibility to Bt toxin Cry1Ac in pink bollworm, Pectinophora gossypiella

Pesticide Biochemistry and Physiology
Shengyun LiXianchun Li

Abstract

Extensive planting of transgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) has spurred increasingly rapid evolution of resistance in pests. In the pink bollworm, Pectinophora gossypiella, a devastating global pest, resistance to Bt toxin Cry1Ac produced by transgenic cotton is linked with mutations in a gene (PgCad1) encoding a cadherin protein that binds Cry1Ac in the larval midgut. We previously reported a long non-coding RNA (lncRNA) in intron 20 of cadherin alleles associated with both resistance and susceptibility to Cry1Ac. Here we tested the hypothesis that reducing expression of this lncRNA decreases transcription of PgCad1 and susceptibility to Cry1Ac. Quantitative RT-PCR showed that feeding susceptible neonates small interfering RNAs (siRNAs) targeting this lncRNA but not PgCad1 decreased the abundance of transcripts of both the lncRNA and PgCad1. Moreover, neonates fed the siRNAs had lower susceptibility to Cry1Ac. The results imply that the lncRNA increases transcription of PgCad1 and susceptibility of pink bollworm to Cry1Ac. The results suggest that disruption of lncRNA expression could be a novel mechanism of pest resistance to Bt toxins.

References

Mar 10, 2001·Journal of Economic Entomology·Y B LiuA C Bartlett
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Shai MorinBruce E Tabashnik
Jul 22, 2004·European Journal of Biochemistry·Juan L Jurat-Fuentes, Michael J Adang
Aug 11, 2005·Nucleic Acids Research·Xiaoyu LinYu Shen
Jun 20, 2008·Genome Research·Marcel E DingerJohn S Mattick
Jan 1, 2010·Nature Reviews. Drug Discovery·Aimee L Jackson, Peter S Linsley
Jul 20, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Fu YangShu-han Sun
Dec 14, 2011·Circulation Research·Aibin HeWilliam T Pu
Jun 5, 2012·Nature Structural & Molecular Biology·Sònia GuilManel Esteller
Jun 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Haonan ZhangYidong Wu
Nov 13, 2015·Scientific Reports·Jeffrey A FabrickBruce E Tabashnik
Nov 20, 2015·PLoS Genetics·Bruce E Tabashnik

❮ Previous
Next ❯

Citations

May 30, 2019·Pest Management Science·Jeffrey A FabrickBruce E Tabashnik
Dec 12, 2020·Frontiers in Genetics·Feng HongDong Wei
May 7, 2021·Science Advances·Xin YangYoujun Zhang
Aug 28, 2021·International Journal of Molecular Sciences·Zhongyuan DengXianchun Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.