A longitudinal study to characterize the distribution patterns of Mycobacterium avium ssp. paratuberculosis in semen, blood and faeces of a naturally infected bull by IS 900 semi-nested and quantitative real-time PCR

Transboundary and Emerging Diseases
Pia MünsterC-P Czerny

Abstract

Johne's disease is caused by Mycobacterium avium ssp. paratuberculosis (MAP) and has been recognized as an important bacterial infection in ruminants. Although MAP has been detected in semen and within the reproductive organs of bulls, the bacterial distribution and shedding patterns are currently not well characterized. Our investigation was performed to detect and quantify MAP in faeces, semen and blood samples repeatedly drawn from a naturally infected but asymptomatic 18-month-old German Simmental breeding bull candidate over a period of 3 years (June 2007-November 2010). Qualitative and quantitative polymerase chain reaction (PCR) techniques were used to correlate the presence and matrix-specific amounts of MAP. In total, 65 sampling dates were selected. Mycobacterium avium ssp. paratuberculosis was detected intermittently in all matrices with MAP-free intervals of up to 18 weeks by an IS900 semi-nested PCR. The number of MAP-positive results from semen and blood samples was higher than from faecal samples. A quantitative polymerase chain reaction detected the highest MAP contents in faeces (10(3) -10(6) MAP/g), while lower amounts were found in semen and blood samples (10(2) -10(5) MAP/ml). Although no significant agreeme...Continue Reading

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Citations

Oct 12, 2012·Tropical Animal Health and Production·Mohamed SalemGeorge Baljer
Apr 27, 2017·Journal of Medical Primatology·Kim FechnerJenny Schäfer
Aug 21, 2019·Veterinary Research·Caroline S CorbettHerman W Barkema
Jan 4, 2018·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Kim FechnerClaus-Peter Czerny
Aug 28, 2020·Veterinary Sciences·Marco RollerAhmed Abd El Wahed

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