A look at the effect of sequence complexity on pressure destabilisation of DNA polymers

Biophysical Chemistry
Gamal Rayan, Robert B Macgregor

Abstract

Our previous studies on the helix-coil transition of double-stranded DNA polymers have demonstrated that molar volume change (ΔV) accompanying the thermally-induced transition can be positive or negative depending on the experimental conditions, that the pressure-induced transition is more cooperative than the heat-induced transition [Rayan and Macgregor, J Phys Chem B2005, 109, 15558-15565], and that the pressure-induced transition does not occur in the absence of water [Rayan and Macgregor, Biophys Chem, 2009, 144, 62-66]. Additionally, we have shown that ΔV values obtained by pressure-dependent techniques differ from those obtained by ambient pressure techniques such as PPC [Rayan et al. J Phys Chem B2009, 113, 1738-1742] thus shedding light on the effects of pressure on DNA polymers. Herein, we examine the effect of sequence complexity, and hence cooperativity on pressure destabilisation of DNA polymers. Working with Clostridium perfringes DNA under conditions such that the estimated ΔV of the helix-coil transition corresponds to -1.78 mL/mol (base pair) at atmospheric pressure, we do not observe the pressure-induced helix-coil transition of this DNA polymer, whereas synthetic copolymers poly[d(A-T)] and poly[d(I-C)] underg...Continue Reading

References

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Jul 21, 2009·Biophysical Chemistry·Gamal Rayan, Robert B Macgregor

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Citations

Jul 25, 2020·Physical Chemistry Chemical Physics : PCCP·Hsuan-Lei Sung, David J Nesbitt
Dec 17, 2019·The Journal of Physical Chemistry. B·Hsuan-Lei Sung, David J Nesbitt

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