Abstract
Our previous studies on the helix-coil transition of double-stranded DNA polymers have demonstrated that molar volume change (ΔV) accompanying the thermally-induced transition can be positive or negative depending on the experimental conditions, that the pressure-induced transition is more cooperative than the heat-induced transition [Rayan and Macgregor, J Phys Chem B2005, 109, 15558-15565], and that the pressure-induced transition does not occur in the absence of water [Rayan and Macgregor, Biophys Chem, 2009, 144, 62-66]. Additionally, we have shown that ΔV values obtained by pressure-dependent techniques differ from those obtained by ambient pressure techniques such as PPC [Rayan et al. J Phys Chem B2009, 113, 1738-1742] thus shedding light on the effects of pressure on DNA polymers. Herein, we examine the effect of sequence complexity, and hence cooperativity on pressure destabilisation of DNA polymers. Working with Clostridium perfringes DNA under conditions such that the estimated ΔV of the helix-coil transition corresponds to -1.78 mL/mol (base pair) at atmospheric pressure, we do not observe the pressure-induced helix-coil transition of this DNA polymer, whereas synthetic copolymers poly[d(A-T)] and poly[d(I-C)] underg...Continue Reading
References
Aug 1, 1977·Biopolymers·S A Hawley, R M Macleod
Aug 1, 1990·Journal of Biomolecular Structure & Dynamics·A T KarapetianM D Frank-Kamenetskii
Sep 1, 1987·Biopolymers·L A Marky, K J Breslauer
Jan 1, 1974·Biopolymers·S A Hawley, R M MacLeod
Mar 1, 1972·Biopolymers·T E Gunter, K K Gunter
Jan 4, 1966·Biochimica Et Biophysica Acta·B Weida, S J Gill
Dec 1, 1966·Biopolymers·F Hughes, R F Steiner
Apr 1, 1995·Biopolymers·J Q Wu, R B Macgregor
Nov 23, 1993·Biochemistry·J Q Wu, R B Macgregor
May 15, 1993·Analytical Biochemistry·J Q Wu, R B Macgregor
Mar 1, 1993·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·Y Z Chen, E W Prohofsky
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·T V ChalikianK J Breslauer
Sep 20, 2001·Journal of the American Chemical Society·D N DubinsT V Chalikian
May 2, 2002·Biochimica Et Biophysica Acta·Robert B Macgregor
Jan 24, 2006·Biopolymers·Peter J Mikulecky, Andrew L Feig
Jul 21, 2006·The Journal of Physical Chemistry. B·Gamal Rayan, Robert B Macgregor
Oct 10, 2006·Biophysical Chemistry·M C LinR B Macgregor
Jul 10, 2007·Nucleic Acids Research·Eric GirardRoger Fourme
Jan 23, 2009·The Journal of Physical Chemistry. B·Gamal RayanHeiko Heerklotz
Jul 21, 2009·Biophysical Chemistry·Gamal Rayan, Robert B Macgregor
Citations
Mar 10, 2020·Chirality·Luboš Plamitzer, Petr Bouř
Jul 25, 2020·Physical Chemistry Chemical Physics : PCCP·Hsuan-Lei Sung, David J Nesbitt
Dec 17, 2019·The Journal of Physical Chemistry. B·Hsuan-Lei Sung, David J Nesbitt