A Look into the Melting Pot: The mecC-Harboring Region Is a Recombination Hot Spot in Staphylococcus stepanovicii

PloS One
Torsten SemmlerBirgit Walther

Abstract

Horizontal gene transfer (HGT) is an important driver for resistance- and virulence factor accumulation in pathogenic bacteria such as Staphylococcus aureus. Here, we have investigated the downstream region of the bacterial chromosomal attachment site (attB) for the staphylococcal cassette chromosome mec (SCCmec) element of a commensal mecC-positive Staphylococcus stepanovicii strain (IMT28705; ODD4) with respect to genetic composition and indications of HGT. S. stepanovicii IMT28705 was isolated from a fecal sample of a trapped wild bank vole (Myodes glareolus) during a screening study (National Network on "Rodent-Borne Pathogens") in Germany. Whole genome sequencing (WGS) of IMT28705 together with the mecC-negative type strain CM7717 was conducted in order to comparatively investigate the genomic region downstream of attB (GenBank accession no. KR732654 and KR732653). The bank vole isolate (IMT28705) harbors a mecC gene which shares 99.2% nucleotide (and 98.5% amino acid) sequence identity with mecC of MRSA_LGA251. In addition, the mecC-encoding region harbors the typical blaZ-mecC-mecR1-mecI structure, corresponding with the class E mec complex. While the sequences downstream of attB in both S. stepanovicii isolates (IMT2870...Continue Reading

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Citations

Jul 8, 2016·Revista Da Sociedade Brasileira De Medicina Tropical·Caio Fernando de OliveiraJohanna Ericson Sollid
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Feb 15, 2020·Expert Review of Anti-infective Therapy·Karsten BeckerHolger Rohde

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Datasets Mentioned

BETA
AB498756.1

Methods Mentioned

BETA
restriction modification

Software Mentioned

Blast
blastn
blastx
PSI
RAST Server
Prodigal Prokaryotic Dynamic Programming Genefinding Algorithm

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