A loop-mediated isothermal amplification assay for the detection and quantification of JC polyomavirus in cerebrospinal fluid: a diagnostic and clinical management tool and technique for progressive multifocal leukoencephalopathy

Virology Journal
Hitomi KinoshitaMasayuki Saijo

Abstract

JC polyomavirus (JCV) is the causative agent of progressive multifocal leukoencephalopathy (PML), a demyelinating disease of the central nervous system in immunosuppressed patients. PML usually has a poor prognosis. Detection and quantification of the JCV genome in cerebrospinal fluid (CSF) is an efficacious tool for the diagnosis and management of PML, for which proper therapeutic interventions are required. A loop-mediated isothermal amplification (LAMP) assay was applied for the quantitative detection of JCV. The LAMP assay was evaluated for the efficacy in diagnosis of PML in comparison with the TaqMan-based quantitative real-time PCR (qPCR) assay using 153 CSF specimens collected from patients with suspected PML. The LAMP assay showed no cross-reactivity against other polyomavirus plasmids, viral DNA, and viral RNA, which causes encephalitis, and detected 1 copy of the standard DNA per reaction. Among 50 qPCR-positives, 42 specimens (containing JCV genome ranged from 3.2 × 100 to 3.2 × 106 copies/reaction) showed positive reactions and 8 specimens (containing 0.9 to 19.9 copies/reaction) showed negative in the LAMP assay. Furthermore, 3 of 103 qPCR-negative specimens showed positive reactions in the LAMP assay. The sensiti...Continue Reading

References

Dec 1, 1980·Journal of Virology·P M HowleyK K Takemoto
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·C SugimotoY Yogo
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Oct 14, 2000·Journal of Neurology, Neurosurgery, and Psychiatry·H Manji, R F Miller
Nov 29, 2001·Journal of Clinical Microbiology·D M WhileyT P Sloots
Feb 16, 2002·Journal of Molecular Evolution·Chie SugimotoYoshiaki Yogo
May 29, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Darío García De ViedmaEmilio Bouza
Jul 30, 2002·Molecular and Cellular Probes·K NagamineT Notomi
May 28, 2004·Journal of Biochemical and Biophysical Methods·Yasuyoshi MoriTsugunori Notomi
Feb 17, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Simona BossolascoPaola Cinque
Aug 10, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Adrienne L McNeesJanet S Butel
Jan 10, 2006·Virology Journal·Moti L ChapagainVivek R Nerurkar
Mar 18, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Laïla SehbaniPatrick Goubau
May 9, 2006·Journal of Virological Methods·Amal ElfaitouriJonas Blomberg
Feb 23, 2007·Journal of Clinical Microbiology·Bipin Raj BistaAbhay Vats
Mar 23, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Masaru IhiraTetsushi Yoshikawa
Nov 15, 2007·Current Neurology and Neuroscience Reports·Joseph R Berger
Feb 26, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Ting CaiZhonghua Meng
Jul 29, 2008·Neurologic Clinics·Thomas Weber
Mar 5, 2009·Antimicrobial Agents and Chemotherapy·Margot BrickelmaierLeonid Gorelik
Oct 12, 2010·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Joseph R Berger
Dec 7, 2010·Nature Reviews. Neurology·Bruce J BrewAvindra Nath
Jan 14, 2011·The Journal of Infectious Diseases·Martyn K White, Kamel Khalili
Jun 21, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Hans H HirschCeline Leboeuf

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Datasets Mentioned

BETA
AB038252
AB038250

Methods Mentioned

BETA
PCR
nucleic acid amplification
electrophoresis

Software Mentioned

320C
CLUSTAL
Primer Explorer
LA
LAMP

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