Abstract
There is continual demand for animal models that allow a quantitative assessment of angiogenic properties of biomaterials, therapies, and pharmaceuticals. In its simplest form, this is done by subcutaneous material implantation and subsequent vessel counting which usually omits spatial data. We have refined an implantation model and paired it with a computational analytic routine which outputs not only vessel count but also vessel density, distribution, and vessel penetration depth, that relies on a centric vessel as a reference point. We have successfully validated our model by characterizing the angiogenic potential of a fibrin matrix in conjunction with recombinant human vascular endothelial growth factor (rhVEGF165). The inferior epigastric vascular pedicles of rats were sheathed with silicone tubes, which were subsequently filled with 0.2 ml of fibrin and different doses of rhVEGF165, centrically embedding the vessels. Over 4 weeks, tissue samples were harvested and subsequently immunohistologically stained and computationally analyzed. The model was able to detect variations over the angiogenic potentials of growth factor spiked fibrin matrices. Adding 20 ng of rhVEGF165 resulted in a significant increase in vasculature w...Continue Reading
References
Dec 25, 2002·Microscopy Research and Technique·Jie LiRobert S Kirsner
Oct 28, 2003·Plastic and Reconstructive Surgery·Yoshio TanakaWayne A Morrison
Jan 7, 2004·Plastic and Reconstructive Surgery·Kevin J CroninWayne A Morrison
Feb 18, 2004·The Journal of Clinical Investigation·Clare R OzawaHelen M Blau
Aug 8, 2006·Tissue Engineering·Ulrich KneserRaymund E Horch
Sep 23, 2006·Journal of Cellular and Molecular Medicine·K Norrby
Nov 2, 2006·Genome Biology·Anne E CarpenterDavid M Sabatini
Jun 1, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Wolfgang MichlitsSeyedhossein Aharinejad
Jun 5, 2007·Microvascular Research·E PolykandriotisU Kneser
Sep 1, 2007·Molecular Medicine·Andreas ArkudasUlrich Kneser
Dec 7, 2007·The American Journal of Pathology·John A RophaelGeraldine M Mitchell
May 23, 2008·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Rainer MittermayrHeinz Redl
Mar 24, 2009·Methods in Molecular Biology·Katherine M Malinda
Jun 6, 2009·Tissue Engineering. Part B, Reviews·Michael LovettDavid L Kaplan
Jul 1, 2009·International Journal of Experimental Pathology·Carolyn A StatonNicola J Brown
Nov 27, 2009·Microscopy Research and Technique·Krista L SiderJohn E Davies
Mar 3, 2010·The Journal of Experimental Medicine·Iris HelfrichDirk Schadendorf
Apr 26, 2011·Methods in Molecular Biology·V Poulaki
Mar 1, 2012·Biomaterials·Daniel J TilkornGeraldine M Mitchell
Dec 13, 2012·Stem Cells and Development·Heidi E LiljaKeren M Abberton
Aug 20, 2014·Tissue Engineering. Part a·Gregor BuehrerAndreas Arkudas
Oct 27, 2016·Physics in Medicine and Biology·K M MeiburgerF Molinari
Dec 17, 2017·Advanced Drug Delivery Reviews·P HeherP Slezak