A low-cost medium for mannitol production by Lactobacillus intermedius NRRL B-3693

Applied Microbiology and Biotechnology
Badal C Saha

Abstract

The production of mannitol by Lactobacillus intermedius NNRL B-3693 using molasses as an inexpensive carbon source was evaluated. The bacterium produced mannitol (104 g/l) from molasses and fructose syrups (1:1; total sugars, 150 g/l; fructose:glucose 4:1) in 16 h. Several kinds of inexpensive organic and inorganic nitrogen sources and corn steep liquor were evaluated for their potential to replace more expensive nitrogen sources derived from Bacto-peptone and yeast extract. Soy peptone D (5 g/l) and corn steep liquor (50 g/l) were found to be suitable substitutes for Bacto-peptone (5 g/l) and Bacto-yeast extract (5 g/l), respectively. The bacterium produced 105 g mannitol per liter from the molasses and fructose syrup (1:1, total sugars 150 g/l; fructose:glucose 4:1) in 22 h using a combination of soy peptone D (5 g/l) and corn steep liquor (50 g/l). This is the first report on the production of mannitol by fermentation using molasses and corn steep liquor.

References

Aug 17, 2000·Immunologic Research·V K SinghS S Agarwal
Apr 18, 2003·Biotechnology and Bioengineering·Badal C Saha, Lawrence K Nakamura
Jun 7, 2003·Biotechnology Progress·F Niklas W von WeymarnMatti S A Leisola

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Citations

Jan 17, 2007·Applied Microbiology and Biotechnology·Xiuping HeBorun Zhang
Jul 7, 2009·Applied Microbiology and Biotechnology·Seung Hoon Song, Claire Vieille
Feb 25, 2010·Applied Microbiology and Biotechnology·Vicente MonederoMaría J Yebra
Nov 11, 2010·Applied Microbiology and Biotechnology·Badal C Saha, F Michael Racine
Feb 22, 2012·Applied Microbiology and Biotechnology·Maria Eugenia OrtizFernanda Mozzi
Apr 23, 2013·Applied Microbiology and Biotechnology·Maria Eugenia OrtizFernanda Mozzi
Jul 4, 2012·Bioprocess and Biosystems Engineering·Laxman S SavergaveVitthal V Jogdand
Sep 8, 2010·Journal of Industrial Microbiology & Biotechnology·Florbela CarvalheiroFrancisco M Gírio
Jun 9, 2014·Journal of Bioscience and Bioengineering·Shamlan M S ReshamwalaArvind M Lali
Jul 1, 2008·Microbial Biotechnology·Sergio Sanchez, Arnold L Demain
Nov 2, 2014·Applied Microbiology and Biotechnology·Hironaga AkitaTamotsu Hoshino
Apr 3, 2010·Applied Microbiology and Biotechnology·Badal C Saha, F Michael Racine
Jul 4, 2019·Applied Microbiology and Biotechnology·Fuzhi LuWanmeng Mu
May 17, 2017·Journal of Industrial Microbiology & Biotechnology·Min ZhangPingkai Ouyang

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