A low dispersion geometry for microchip separation devices

Analytical Chemistry
D Dutta, D T Leighton

Abstract

Curved channel geometries introduced on microchip separation devices to achieve greater separation distances often lead to large analyte dispersion, degrading the performance of these systems. While such electrokinetic dispersion may be minimized by reducing the channel width around the curved region, alternative strategies involving larger channel curvatures may be promising as well, depending on the application. For example, Culbertson et al. (Anal. Chem. 2000, 72, 5814-5819) recently demonstrated the effectiveness of gentle spiral geometries in carrying out separations of small molecules. For moderate and large Peclet number systems, however, larger spiral geometries are necessary to diminish electrokinetic dispersion of solute slugs which may not conform to the needs of the microchip format. In this work, we investigate a modified spiral geometry with a wavy wall along the inner track of the channel. Analysis shows that such width profiling may significantly improve the performance of the spiral geometry, making the design effective for larger Peclet number or smaller radii systems. Numerical simulations performed to optimize these modified spirals suggest equating transit times along the inner and the outer track of the ch...Continue Reading

References

Apr 1, 1995·Electrophoresis·S C Jacobson, J M Ramsey
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·L BousseJ W Parce
Aug 22, 2000·Analytical Chemistry·S V ErmakovJ M Ramsey
Feb 24, 2001·Analytical Chemistry·S K Griffiths, R H Nilson

❮ Previous
Next ❯

Citations

Jun 8, 2007·Journal of Forensic Sciences·Katie M HorsmanJames P Landers
Nov 23, 2006·Electrophoresis·Angela De Leebeeck, David Sinton
Jun 21, 2007·Electrophoresis·David E Huber, Juan G Santiago
Feb 20, 2013·The Analyst·Ling Xia, Debashis Dutta
Sep 26, 2012·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Debashis Pal, Suman Chakraborty
Apr 30, 2005·Analytical Chemistry·J J Kirchner, E F Hasselbrink
Apr 9, 2009·Analytical Chemistry·Adarsh D RadadiaMark A Shannon
Jul 2, 2002·Analytical Chemistry·Darwin R ReyesAndreas Manz
Nov 1, 2005·Analytical Chemistry·Andrew J SkulanGregory J Fiechtner
Dec 17, 2003·Analytical Chemistry·Gregory J Fiechtner, Eric B Cummings

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.