PMID: 2110056Mar 30, 1990Paper

A low-molecular-mass Kazal-type protease inhibitor isolated from rat hepatocytes is identical to rat pancreatic secretory trypsin inhibitor II. Purification and amino acid sequence

European Journal of Biochemistry
H KidoN Katunuma

Abstract

A low-molecular-mass serine protease inhibitor was purified from hepatocytes and liver of rats. It was found to be a single polypeptide of 56 amino acid residues corresponding to Mr = 6224, a value that is in agreement with the molecular mass determined by gel chromatography. The inhibitor formed a complex in a molar ratio of 1:1 with trypsin. Its complete amino acid sequence was identical with that of pancreatic secretory trypsin inhibitor II (PSTI-II) in pancreatic juice, but not with that of PSTI-I [Uda, K., Ogawa, M., Shibata, T., Murata, A., Mori, T., Kikuchi, N., Yoshida, N., Tsunasawa, S. & Sakiyama, F. (1988) Biol. Chem. Hoppe-Seyler 369, 55-61]. PSTIs have been reported to be primarily pancreatic secretory products, but in have been reported to be primarily pancreatic secretory products, but in patients immunoreactive PSTI was found in the plasma and urine during acute inflammatory disease and shown to be produced ectopically in cancer tissues. Here we report for the first time that PSTI-II is present in other normal tissues besides the pancreas.

References

Dec 1, 1979·European Journal of Biochemistry·T TowatariN Katunuma
Jul 14, 1989·Biochemical and Biophysical Research Communications·A HoriiK Matsubara
Jan 1, 1988·Biological Chemistry Hoppe-Seyler·G PohlentzK Sandhoff
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Sep 1, 1948·Journal of the American Chemical Society·L A KAZALR A BRAHINSKY

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Citations

Mar 1, 1993·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·A E AriasM Bendayan
Oct 12, 2012·PloS One·Aurelie D DesgardinJohn M Cunningham
Apr 24, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mariola SłowińskaAndrzej Ciereszko
Jan 1, 1991·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·U H StenmanO Itkonen
Dec 21, 2002·The Journal of Biological Chemistry·Henry Lardy
Sep 10, 1999·Journal of Chromatography. a·A LopuskaA Polanowski

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