A low molecular weight DNA polymerase from wheat embryos

Plant Molecular Biology
M CastroviejoS Litvak

Abstract

The study of plant DNA polymerases lags far behind that concerning their animal or yeast counterpart. In this work we describe the first extensive purification to apparent homogeneity, as well as a detailed biochemical and immunological characterization, of a low molecular weight DNA polymerase (DNA polymerase CI) purified from wheat embryos. The monomeric enzyme is a basic protein having a molecular weight of 52 kDa. Polyclonal antibodies raised in rabbits against DNA polymerase CI did not inhibit animal DNA polymerases alpha and beta or wheat DNA polymerase A, whereas wheat DNA polymerases CII and B were much less affected than the CI enzyme. Several properties of enzyme CI were studied. Some known inhibitors of DNA polymerase activity including aphidicolin, phosphonoacetic acid and heparin, did not affect DNA polymerase CI while the activity of this enzyme was strongly inhibited by ddTTP and N-ethylmaleimide. The polyamine spermine decreased markedly the enzyme activity, while spermidine produced a strong stimulation at the same concentrations that spermine inhibited the enzyme. The best template for this enzyme is poly dA-oligo dT, although polymerase CI can recognize significantly some synthetic polyribonucleotide template...Continue Reading

References

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Citations

Dec 1, 1992·Plant Molecular Biology·P CoelloJ M Vázquez-Ramos
Sep 1, 1996·Plant Molecular Biology·J P BenedettoM Castroviejo
Feb 1, 1992·Molecular Biology Reports·T S BalmukhanovN A Ajtkhozhina
Jun 23, 2004·Biochemical and Biophysical Research Communications·Sailendra Nath SarkarDibyendu N Sengupta
May 15, 1991·Archives of Biochemistry and Biophysics·M C RichardM Castroviejo
Nov 5, 2003·Journal of Plant Physiology·Edurne Gómez Roig, Jorge M Vázquez-Ramos

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