A low molecular weight insulin-like growth factor binding protein from rat: cDNA cloning and tissue distribution of its messenger RNA.

Molecular Endocrinology
J B MargotJ Schwander

Abstract

Rat serum contains two major forms of insulin-like growth factor (IGF) binding proteins (BPs) that have apparent mol wts of about 35,000 and 150,000. We have isolated a cDNA clone encoding an IGF-BP whose N-terminal sequence is completely homologous to the NH2-terminal of the Buffalo rat liver cells-3A BP. The 270 amino acid mature protein has a predicted mol wt of 29,500. It contains a cysteine rich domain at each end of the molecule and an Arg-Gly-Asp (RGD) tripeptide motif near its C-terminus which suggests that this BP might associate with integrin cell surface receptors. The mature protein shares only partial homology with two published human IGF-BPs. Northern blot analysis shows that its mRNA is abundant in several fetal tissues, in adult brain, testes, ovaries, and kidney. Expression in the liver is high in fetal life but decreases to a barely detectable level in adulthood. However, upon hypophysectomy, the mRNA level increases at least 20-fold which suggests a hormonal regulation for the hepatic production of this small IGF-BP.

Citations

Jan 1, 1994·Journal of Cancer Research and Clinical Oncology·J HofmannK Havemann
Jul 1, 1991·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·W F BlumO Mehls
Jan 1, 1992·Breast Cancer Research and Treatment·R F Krywicki, D Yee
Jan 1, 1992·Breast Cancer Research and Treatment·J A Figueroa, D Yee
Mar 1, 1991·Breast Cancer Research and Treatment·D YeeD R Powell
Feb 5, 2005·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Burkhard TönshoffSonia Ciarmatori
May 23, 1998·In Vitro Cellular & Developmental Biology. Animal·D G Matsell, T Bennett
Dec 1, 1989·Molecular and Cellular Endocrinology·S L Drop
May 1, 1991·Molecular and Cellular Endocrinology·M S Lewitt, R C Baxter
Aug 1, 1994·Molecular and Cellular Endocrinology·A G SchullerS L Drop
Feb 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·K A KampmanM E White
May 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·A R AldredG Schreiber
Jul 1, 1994·Comparative Biochemistry and Physiology. Biochemistry and Molecular Biology·K A KampmanM E White
Jul 1, 1995·Domestic Animal Endocrinology·L J Spicer, S E Echternkamp
Mar 19, 1999·Domestic Animal Endocrinology·L J Spicer, C S Chamberlain
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·C DuanK Pozios
Jan 1, 1991·Cancer Investigation·K J CullenN Rosen
Jan 12, 2000·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·M R CorkinsR G MacDonald
Jun 1, 1996·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·D R PowellR L Hintz
Feb 1, 2008·Reproductive Biology and Endocrinology : RB&E·Steven D FlemingStephen C Bell
Dec 30, 2014·Cytokine & Growth Factor Reviews·V C RussoG A Werther
Apr 16, 2013·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Adam P SharplesClaire E Stewart
Jun 28, 1991·Biochemical and Biophysical Research Communications·S K SprattA Sommer
Jan 30, 1990·Biochemical and Biophysical Research Communications·A L Albiston, A C Herington
Jan 1, 1991·Annals of the New York Academy of Sciences·S C Bell
Jan 1, 1991·Annals of the New York Academy of Sciences·L C GiudiceJ C Irwin

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