A LuxP-based fluorescent sensor for bacterial autoinducer II

ACS Chemical Biology
Jinge Zhu, Dehua Pei

Abstract

Autoinducer 2 (AI-2), which enables different bacterial species to engage in interspecies communication, has been difficult to detect quantitatively. Currently, the most commonly used method for AI-2 detection employs an engineered Vibrio harveyi reporter strain, which produces bioluminescence in response to AI-2. However, the bioassay is not quantitative and is sensitive to assay conditions. In this work, we have developed two protein sensors for AI-2 by modifying AI-2 receptor proteins LuxP and LsrB with environmentally sensitive fluorescent dyes. The protein sensors bind specifically to AI-2 and produce dose-dependent changes in their fluorescence yield. The new assay method has been applied to monitor the enzymatic synthesis of AI-2 in real time and determine the extracellular and intracellular AI-2 concentrations in several bacterial culture fluids.

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Citations

Feb 19, 2016·Angewandte Chemie·Kyoji TsuchikamaKim D Janda
Dec 8, 2009·Trends in Biotechnology·Galen S LovingBarbara Imperiali
Jun 6, 2015·The ISME Journal·Matthew D ServinskyWilliam E Bentley
Feb 10, 2009·Journal of Food Science·Bassam A AnnousJames L Smith
Feb 6, 2015·Analytical Chemistry·Nilesh RautSylvia Daunert
Jun 9, 2009·Journal of the American Chemical Society·Shinya TsukijiItaru Hamachi
May 10, 2017·Critical Reviews in Microbiology·Elio RossiUte Römling
Sep 16, 2018·Bulletin of Mathematical Biology·Majid Latif, Elebeoba E May
Nov 3, 2011·Current Protocols in Microbiology·Michiko E Taga, Karina B Xavier
Oct 25, 2020·Nature Communications·Lei ZhangXihui Shen
Dec 21, 2019·Journal of Theoretical Biology·Pei Yen TanYu Liu

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