PMID: 9420221Jan 7, 1998Paper

A lymph node-derived cytopathic simian immunodeficiency virus Mne variant replicates in nonstimulated peripheral blood mononuclear cells

Journal of Virology
J T KimataJ Overbaugh

Abstract

Lymph nodes (LNs) are sites of active human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) replication and disease at both early and late stages of infection. Consequently, variant viruses that replicate efficiently and subsequently cause immune dysfunction may be harbored in this tissue. To determine whether LN-associated SIVs have an increased capacity to replicate and induce cytopathology, a molecular clone of SIV was isolated directly from DNA extracted from unpassaged LN tissue of a pig-tailed macaque (Macaca nemestrina) infected with SIVMne. The animal had declining CD4+ T-lymphocyte counts at the time of the LN biopsy. In human CD4+ T-cell lines, the LN-derived virus, SIVMne027, replicated with relatively slow kinetics and was minimally cytopathic and non-syncytium inducing compared to other SIVMne clones. However, in phytohemagglutinin-stimulated pig-tailed macaque peripheral blood mononuclear cells (PBMCs), SIVMne027 replicated efficiently and was highly cytopathic for the CD4+ T-cell population. Interestingly, unlike other SIVMne clones, SIVMne027 also replicated to a high level in nonstimulated macaque PBMCs. High-level replication depended on the presence of both the T-cell and monocyt...Continue Reading

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Citations

Mar 27, 2009·AIDS Research and Human Retroviruses·Susan M GrahamJulie Overbaugh
Jan 22, 2013·Biology·Rajesh ThippeshappaJason T Kimata
Jan 18, 2013·Journal of Virology·Julia BitzegeioTheodora Hatziioannou
Aug 31, 2013·PLoS Pathogens·Fan WuVanessa M Hirsch
Jun 2, 2020·Frontiers in Microbiology·Rajesh ThippeshappaDeepak Kaushal
Jan 9, 1999·Journal of Virology·J T KimataJ Overbaugh

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