A lysosomal-mitochondrial death pathway is induced by solamargine in human K562 leukemia cells

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Lingmei SunHongxiang Lou

Abstract

Solamargine (SM), a steroidal alkaloid glycoside from Solanum nigrum L., displayed a superior cytotoxicity to many human tumor cells. Further investigation with human K562 leukemia cells found that SM could induce an early lysosomal rupture within 2h as assessed by acridine-orange relocation and alkalinization of lysosomes. Intracellular lysosomal rupture is also confirmed with the release of cathepsin B to cytosol detected by western blot. Subsequent mitochondrial damage including mitochondrial membrane permeabilization detected by decrease membrane potential as well as the release of cytochrome c from mitochondria was also observed. The cellular Ca(2+) overload is more pronounced in SM-treated cells. Cells exposed to 10 microM SM for 30 min showed a maximum 7-fold increase in intracellular calcium concentration compared with vehicle-treated controls. The down-expression of Bcl-2, up-regulation of Bax, caspase-3 and caspase-9 activities followed by above changes revealed that the cytotoxicity of SM was involved in a lysosomal-mitochondrial death pathway induced by SM.

References

Nov 5, 1997·Biochemical and Biophysical Research Communications·D J McConkey, S Orrenius
Nov 14, 2000·The Journal of Biological Chemistry·V StokaG S Salvesen
Nov 20, 2001·Molecular and Cellular Biology·K OnoJ Han
Jan 10, 2002·Frontiers in Bioscience : a Journal and Virtual Library·Michael R ChoDavid E Golan
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Xi-Ming YuanUlf T Brunk
Apr 22, 2003·Journal of Cellular Biochemistry·Chwen-Ming ShihChien-Tsu Chen
May 6, 2003·Biochemical and Biophysical Research Communications·György HajnóczkyMuniswamy Madesh
Jul 3, 2003·Nature Reviews. Molecular Cell Biology·Sten OrreniusPierluigi Nicotera
Sep 3, 2003·European Journal of Biochemistry·Ming ZhaoUlf T Brunk
Jan 18, 2006·Biochimica Et Biophysica Acta·Claudine TardyNathalie Andrieu-Abadie
Jun 2, 2006·Chemico-biological Interactions·Alexei TermanUlf T Brunk
Feb 3, 2007·Molecular Nutrition & Food Research·Peter G Bradford, Atif B Awad
Jul 10, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·L Y ShiuK W Kuo
Nov 21, 2007·Journal of Ethnopharmacology·Y B JiX Zou

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Citations

Jan 11, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Cheng-Jeng TaiChen-Jei Tai
Jul 15, 2015·Oncology Letters·Xiaodong XieGenbao Shao
May 10, 2011·Journal of Pharmaceutical and Biomedical Analysis·Xiao ZhengGuangji Wang
Mar 31, 2015·Medicinal Research Reviews·Qi-Wei JiangZhi Shi
Feb 14, 2012·Magnetic Resonance in Chemistry : MRC·Maria Conceição M TorresOtília Deusdênia L Pessoa
Mar 6, 2012·Phytotherapy Research : PTR·Shuli ManChangxiao Liu
Feb 19, 2014·Bioorganic & Medicinal Chemistry Letters·Xin ZanHong-Xiang Lou
Jul 20, 2014·Carbohydrate Polymers·Dan LiFuliang Xiong
Jul 30, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Chien-Kai WangChun-Chao Chang
Mar 31, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Iman Karimi SaniFatemeh Kalalinia
Mar 31, 2015·Journal of Agricultural and Food Chemistry·Mendel Friedman
Oct 4, 2011·Journal of Natural Products·Maria Conceição M TorresMarcos Hikari Toyama
Oct 19, 2016·Cancer Biotherapy & Radiopharmaceuticals·Zongyan ChenShumei Ma
Apr 7, 2017·Phytotherapy Research : PTR·Fatemeh Kalalinia, Iman Karimi-Sani
Nov 6, 2018·International Journal of Cancer. Journal International Du Cancer·Prasanta DeyHyung Sik Kim
Sep 1, 2020·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Wenjing LiGe Yang
Dec 17, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Mariza Abreu MirandaMandip Singh

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