A LysR-Type Transcriptional Regulator, RovM, Senses Nutritional Cues Suggesting that It Is Involved in Metabolic Adaptation of Yersinia pestis to the Flea Gut

PloS One
Viveka Vadyvaloo, Angela K Hinz

Abstract

Yersinia pestis has evolved as a clonal variant of Yersinia pseudotuberculosis to cause flea-borne biofilm-mediated transmission of the bubonic plague. The LysR-type transcriptional regulator, RovM, is highly induced only during Y. pestis infection of the flea host. RovM homologs in other pathogens regulate biofilm formation, nutrient sensing, and virulence; including in Y. pseudotuberculosis, where RovM represses the major virulence factor, RovA. Here the role that RovM plays during flea infection was investigated using a Y. pestis KIM6+ strain deleted of rovM, ΔrovM. The ΔrovM mutant strain was not affected in characteristic biofilm gut blockage, growth, or survival during single infection of fleas. Nonetheless, during a co-infection of fleas, the ΔrovM mutant exhibited a significant competitive fitness defect relative to the wild type strain. This competitive fitness defect was restored as a fitness advantage relative to the wild type in a ΔrovM mutant complemented in trans to over-express rovM. Consistent with this, Y. pestis strains, producing elevated transcriptional levels of rovM, displayed higher growth rates, and differential ability to form biofilm in response to specific nutrients in comparison to the wild type. In ...Continue Reading

References

Jan 1, 1993·Annual Review of Microbiology·M A Schell
Mar 1, 1996·Journal of Bacteriology·N SurgeyG Condemine
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·M AchtmanE Carniel
Feb 27, 2003·Molecular Microbiology·Susan A Joyce, David J Clarke
Sep 18, 2003·FEMS Immunology and Medical Microbiology·Anne DerbiseElisabeth Carniel
Mar 5, 2004·Journal of Bacteriology·Robert D PerryJacqueline D Fetherston
Jul 24, 2004·The Journal of Infectious Diseases·Clayton O JarrettB Joseph Hinnebusch
Sep 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·P S G ChainE Garcia
Jul 26, 2006·Proceedings of the National Academy of Sciences of the United States of America·Florent SebbaneB Joseph Hinnebusch
Aug 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jason S CathelynVirginia L Miller
Jun 19, 2007·Journal of Bacteriology·Matthew B Lawrenz, Virginia L Miller
Oct 31, 2007·Advances in Experimental Medicine and Biology·Ann Kathrin HerovenPetra Dersch
Dec 3, 2008·Microbiology·Sarah E Maddocks, Petra C F Oyston
Feb 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Suzanne E OsborneBrian K Coombes
Mar 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·J Christian Perez, Eduardo A Groisman
Mar 21, 2009·PLoS Genetics·J Christian PerezEduardo A Groisman
Jan 20, 2011·Acta Crystallographica. Section D, Biological Crystallography·Nick QuadeDirk W Heinz
Feb 14, 2012·Journal of Bacteriology·Katherine A RempeViveka Vadyvaloo
Mar 13, 2012·Current Opinion in Microbiology·Iman Chouikha, B Joseph Hinnebusch
Jun 12, 2012·Journal of Medical Entomology·Ryan T JonesKenneth L Gage

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Citations

Aug 7, 2016·BMC Microbiology·Zizhong LiuYanping Han
Sep 10, 2017·Annual Review of Microbiology·B Joseph HinnebuschDavid M Bland
Jun 5, 2019·Evolution Letters·Sylvain GandonFlorent Sebbane
Jan 23, 2021·The ISME Journal·Typhanie BouvenotFlorent Sebbane
Feb 7, 2021·Biomolecules·B Joseph HinnebuschDavid M Bland

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Methods Mentioned

BETA
PCR

Software Mentioned

GraphPad Prism
Primer Express

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