PMID: 9185566Jul 1, 1997Paper

A macrocyclic bis-acridine shifts the equilibrium from duplexes towards DNA hairpins

Nucleic Acids Research
A Slama-SchwokJ M Lehn

Abstract

Nucleic acids can undergo dynamic conformational changes associated with the regulation of biological processes. A molecule presenting larger affinities for alternative structures relative to a duplex is expected to modify such conformational equilibria. We have previously reported that macrocyclic bis-acridine binds preferentially to single-stranded regions, especially DNA hairpins, due to steric effects. Here, we show, using gel electrophoresis, fluorescence and melting temperature experiments, that the macrocycle bis-acridine shifts an equilibrium from a duplex towards the corresponding hairpins. Competition experiments enlighten the higher affinity of the macrocycle for hairpins compared with double-stranded DNA. The macrocycle bis-acridine destabilizes a synthetic polynucleotide, by the formation of premelted areas. By extrapolation, the macrocycle bis-acridine should be able to disrupt, at least locally, genomic DNA duplexes and to stabilize unpaired areas, especially palindromic ones forming hairpins. Such macrocyclic compounds may have potential applications in the therapy of diseases involving hairpins.

References

Aug 14, 1992·Science·D J EckerM Zounes
Jul 11, 1992·Nucleic Acids Research·V SégaultC Branlant
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C T McMurrayJ O Douglass
Feb 24, 1989·Science·M E BianchiG Paonessa
May 31, 1988·Biochemical and Biophysical Research Communications·S WagaM Yoshida
Jun 1, 1983·Biopolymers·J Widom, R L Baldwin
May 25, 1983·Journal of Molecular Biology·R E Dickerson
Oct 24, 1981·Nucleic Acids Research·B C Hoopes, W R McClure
Jun 9, 1995·Science·J P MarinoD M Crothers
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·K S Wilson, P H von Hippel
Jan 1, 1995·Bioconjugate Chemistry·D R CoreyA Huang
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·X ChenG Gupta
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·R Tan, A D Frankel
Sep 25, 1994·Nucleic Acids Research·J C FrançoisC Hélène
Jan 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K van Holde, J Zlatanova
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·O D HensensI H Goldberg
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·E SkripkinC Ehresmann
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C SpiroC T McMurray
Mar 14, 1996·Nature·K HaradaA D Frankel
Mar 8, 1996·The Journal of Biological Chemistry·J PeltaJ L Sikorav
Feb 9, 1996·Science·M H WernerG M Clore
Jun 7, 1996·Biochimica Et Biophysica Acta·B BarbeauE Rassart

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Citations

Feb 4, 1998·Biophysical Journal·E RaspaudF Livolant
Jan 1, 1999·Bioorganic & Medicinal Chemistry Letters·A J BlackerJ M Lehn
Jan 16, 2002·Journal of Biomolecular Structure & Dynamics·P AlbertiJ L Mergny
Mar 19, 2014·Chemical Society Reviews·Anton GranzhanMarie-Paule Teulade-Fichou
Jan 1, 2012·Scientifica·Ellen Robertshaw, Clemens Kiecker
Jan 12, 2012·Journal of Skin Cancer·K C MasoumiRamin Massoumi
Oct 28, 2015·Chemistry : a European Journal·Martin Gasiorek, Hans-Jörg Schneider
Dec 9, 2003·Chembiochem : a European Journal of Chemical Biology·Arnaud DavidMarie-Paule Teulade-Fichou
Aug 21, 2010·Journal of Nucleic Acids·Gaëlle Lenglet, Marie-Hélène David-Cordonnier

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