A Magnesium-Enriched 3D Culture System that Mimics the Bone Development Microenvironment for Vascularized Bone Regeneration

Advanced Science
Sihan LinXinquan Jiang

Abstract

The redevelopment/regeneration pattern of amputated limbs from a blastema in salamander suggests that enhanced regeneration might be achieved by mimicking the developmental microenvironment. Inspired by the discovery that the expression of magnesium transporter-1 (MagT1), a selective magnesium (Mg) transporter, is significantly upregulated in the endochondral ossification region of mouse embryos, a Mg-enriched 3D culture system is proposed to provide an embryonic-like environment for stem cells. First, the optimum concentration of Mg ions (Mg2+) for creating the osteogenic microenvironment is screened by evaluating MagT1 expression levels, which correspond to the osteogenic differentiation capacity of stem cells. The results reveal that Mg2+ selectively activates the mitogen-activated protein kinase/extracellular regulated kinase (MAPK/ERK) pathway to stimulate osteogenic differentiation, and Mg2+ influx via MagT1 is profoundly involved in this process. Then, Mg-enriched microspheres are fabricated at the appropriate size to ensure the viability of the encapsulated cells. A series of experiments show that the Mg-enriched microenvironment not only stimulates the osteogenic differentiation of stem cells but also promotes neovascu...Continue Reading

References

Jan 15, 1996·International Journal of Radiation Oncology, Biology, Physics·K Groebe, W Mueller-Klieser
Mar 30, 2002·Science·George M Whitesides, Bartosz Grzybowski
Jun 5, 2003·Nature Medicine·Napoleone FerraraJennifer LeCouter
May 9, 2007·Journal of Colloid and Interface Science·Jingwei Xie, Chi-Hwa Wang
Sep 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hao Zhou, David E Clapham
Mar 24, 2010·Acta Biomaterialia·N C HuntL M Grover
Jul 29, 2011·Nature·Feng-Yen LiMichael J Lenardo
Sep 4, 2012·Trends in Biotechnology·Susmita BoseAmit Bandyopadhyay
Sep 7, 2013·Wiley Interdisciplinary Reviews. Developmental Biology·András Simon, Elly M Tanaka
Sep 10, 2013·Trends in Biotechnology·Susmita BoseAmit Bandyopadhyay
May 21, 2014·Journal of the Mechanical Behavior of Biomedical Materials·Georgia KaklamaniJames Bowen
Mar 31, 2016·Nature Communications·Hibiki Vincent TanakaChikafumi Chiba
Aug 4, 2016·Development·Kishor K Sivaraj, Ralf H Adams
Oct 19, 2016·Trends in Biotechnology·Matthias W Laschke, Michael D Menger
Jun 4, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Livia RosetiBrunella Grigolo

❮ Previous
Next ❯

Citations

Jun 20, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Haotian PanXiaodong Cao
Apr 11, 2020·International Journal of Nanomedicine·Yun'an QingYanguo Qin
Sep 29, 2020·International Journal of Nanomedicine·Xiaodong LiLing Zhang
Nov 6, 2020·Chemistry : a European Journal·Jéssica S BarbosaFilipe A Almeida Paz
Jan 7, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Miao SunHuiming Wang
Dec 18, 2020·Frontiers in Bioengineering and Biotechnology·Qing Zhang
Feb 14, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Xintao ZhangHui Zeng
Feb 25, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xinyun ZhaiWilliam Weijia Lu
May 1, 2021·International Journal of Molecular Sciences·Hung-Jin HuangHui-Wen Chiu
May 27, 2021·Advanced Healthcare Materials·Monika ŠalandováLidy E Fratila-Apachitei
May 29, 2021·Advanced Science·Jinglun ZhangYufeng Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
amputation
electrophoresis
flow cytometry
Flow
gene knockout
reverse
transfection
fluorescence microscopy

Software Mentioned

LDI
SAS
LDI system

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.