A magnet-actuated biomimetic device for isolating biological entities in microwells

Scientific Reports
Himani SharmaAmit Agrawal

Abstract

Microwell platforms show great promise in single-cell studies and protein measurements because of their low volume sampling, rapid analysis and high throughput screening ability. However, the existing actuation mechanisms to manipulate the target samples and fabrication procedures involved in the microwell-based microfluidic devices are complex, resource-intensive and require an external power source. In this work, we present proof of concept of a simple, power-free and low-cost closed magnet digital microfluidics device for isolating biological entities in femtoliter-sized microwells. The target biological entities were encapsulated in magnetic liquid marbles and shuttled back and forth between micropatterned top and bottom plates in the microdevice to obtain high loading efficiency and short processing time. The microdevice performance was studied through fluorescent detection of three different entities: microbeads, bovine serum albumin (BSA) and Escherichia coli, captured in the microwell array. Almost 80% of the microwells were loaded with single microbeads in five shuttling cycles, in less than a minute. Further, a low volume of BSA was compartmentalized in the microwell array over a two order range of concentration. The ...Continue Reading

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Citations

Oct 5, 2019·Advanced Materials·Xiaoxuan ZhangYuanjin Zhao
Jul 2, 2021·Electrophoresis·Hao ChengMing Li

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Methods Mentioned

BETA
ELISA
X-ray
confocal microscopy
Fluorescence
Fluorescence microscopy

Software Mentioned

Surface Evolver
ImageJ

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