A magnetic nanoparticle-labeled immunoassay with europium and samarium for simultaneous quantification of serum pepsinogen I and II

British Journal of Biomedical Science
J FanB Huang

Abstract

To develop a novel immunoassay for the simultaneous determination of serum pepsinogen I (PG I) and pepsinogen II (PG II) by combining established methods of time-resolved fluoroimmunoassay (TRFIA) and magnetic nanoparticles separation. This new immunoassay method was characterised by immobilising primary antibodies on the surface of magnetic particles and labelled with stable fluorescent chelates of europium and samarium. Using magnetic nanoparticles, the TRFIA immunoassay exhibited broad dynamic assay ranges for PG I with detection limit of 0.33 ng/mL, while for PG II with detection limit of 0.38 ng/mL. Cross-reactivity between PGs I and II were <15. The intra- and inter-assay coefficient variations of the method were <3%, and the recoveries were in the range of 97-103% for serum samples. Bland-Altman analysis of 124 serum samples showed good consistency with a commercial TRFIA kit. For PG I, the mean (95% confidence interval) difference was 0.97 (-14.3-12.3) ng/mL, whilst for PG II the difference was 0.6 (-4.4-3.2) ng/mL. Our data suggest that the method is feasible and could be developed into a platform for the routine clinical determination of PG I and PG II levels in human serum.

References

Apr 1, 1987·Gastroenterologia Japonica·K MikiK Takahashi
Mar 1, 1984·Analytical Biochemistry·I HemmiläT Lövgren
May 1, 1995·Pharmacology & Therapeutics·E F DicksonE P Diamandis
Jan 1, 1995·Journal of Photochemistry and Photobiology. B, Biology·E F DicksonE P Diamandis
Feb 17, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·I GrittiG S Roi
Mar 28, 2002·Cellular and Molecular Life Sciences : CMLS·T Kageyama
Sep 2, 2006·Analytical Biochemistry·Tieming HuoChuanlai Xu
Jan 20, 2007·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Kazumasa Miki
Mar 24, 2007·Internal Medicine·Chizu MukoubayashiMasao Ichinose
Apr 12, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Xu WangJin-Ming Lin
Oct 1, 1984·Talanta·T LövgrenE Bertoft
Dec 2, 2008·Tropical Animal Health and Production·James V ConlanLaurence J Gleeson
Feb 21, 2009·World Journal of Gastroenterology : WJG·Katsunori IijimaTooru Shimosegawa
Apr 13, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Fanggui YeShulin Zhao
May 11, 2011·Analytical and Bioanalytical Chemistry·A K Hagan, T Zuchner

❮ Previous
Next ❯

Citations

Oct 19, 2017·British Journal of Biomedical Science·Andrew Blann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.