A major DNA binding protein encoded by BALF2 open reading frame of Epstein-Barr virus (EBV) forms a complex with other EBV DNA-binding proteins: DNAase, EA-D, and DNA polymerase

Virology
Y ZengT Ooka

Abstract

A major 135-kDa DNA binding protein (mDBP) encoded by the BALF2 open reading frame of Epstein-Barr Virus (EBV) is known to be an essential protein for the induction of the lytic cycle. The present investigation was carried out to know whether this protein forms a complex in vivo with other viral DNA binding proteins (DBP) involved in DNA replication: DNA polymerase, EA-D (diffused early antigen), and DNAase. Immunoprecipitation assays followed by mono- and two-dimensional electrophoresis showed that mDBP forms a complex with these three DBP. Other complexes were also found such as EA-D/DNAase, DNA polymerase/DNAase, and DNA polymerase/EA-D. The complexed forms already exist in the early stage of EBV cycle before DNA synthesis is induced in the EBV producer P3HR-1 cell line. The exonuclease activity encoded by DNAase was found to be inhibited when this enzyme complexed with mDBP, while the EBV DNA polymerase retained its activity in the complexed form with mDBP. Our results suggest that these complexes already present before DNA synthesis are necessary for EBV DNA synthesis.

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Citations

Sep 26, 2013·Clinical & Developmental Immunology·Anette Holck DraborgGunnar Houen
Jul 20, 2012·Clinical & Developmental Immunology·Anette Holck DraborgGunnar Houen
Jan 1, 2010·Future Virology·Andrew J Rennekamp, Paul M Lieberman
Dec 17, 2002·Critical Reviews in Oncology/hematology·Jaap M MiddeldorpChris J L M Meijer
Jun 23, 2009·Journal of Molecular Biology·Marlyse BuissonWim P Burmeister
Sep 15, 2007·Journal of Medical Virology·Dewi K ParamitaJaap M Middeldorp
Apr 11, 2007·Advances in Cancer Research·George MillerLyn Gradoville
Nov 10, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Jie XuQuentin Liu
Aug 11, 2017·The Journal of General Virology·Ria GoswamiEdward Gershburg
Feb 8, 2019·Scandinavian Journal of Clinical and Laboratory Investigation·Louise SternbækGunnar Houen
Dec 12, 2019·Proceedings of the National Academy of Sciences of the United States of America·Lijun JiangKa-Leung Wong

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