PMID: 7528203Jul 1, 1994Paper

A major glucocorticoid-inducible P450 in rat liver is not P450 3A1

Journal of Biochemistry
M Komori, Y Oda

Abstract

A new P450 3A cDNA (RL33) has been cloned from a liver cDNA library of untreated male rat. RL33 is 2032 nucleotides in length and has an open reading frame of 502 amino acid residues. The nucleotide sequence of its 5'-noncoding region is completely identical with that of a genomic clone of P450 3A1 isolated by Burger et al. [Proc. Natl. Acad. Sci. USA 89, 2145-2149 (1992)]. Compared with rat P450 3A1, P450 RL33 showed 98 and 97% identities in the nucleotide and deduced amino acid sequences, respectively, with the deletion of 2 amino acids and substitution of 12 amino acids. These residues were localized around amino acids 107-230. Recently Kirita and Matsubara have isolated the same P450 3A cDNA (cDEX) from dexamethasone (DEX)-treated rat liver [Arch. Biochem. Biophys. 307, 253-258 (1993)]. Northern blot analysis using an oligonucleotide probe specific for P450 RL33/cDEX revealed that P450 RL33/cDEX mRNA was induced strongly by pregnenolone 16 alpha-carbonitrile and DEX and weakly by phenobarbital (PB) and triacetyloleandomycin. We constructed a P450 3A cDNA library by the reverse transcriptase-polymerase chain reaction using common primers to P450 RL33/cDEX, 3A1, and 3A2, and subcloned the cDNAs into pUC119. The expression lev...Continue Reading

Citations

May 1, 1996·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·R L CheungD S Riddick
Jun 20, 1998·The Journal of Biological Chemistry·J M Huss, C B Kasper
Apr 18, 2002·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·G G GibsonW El-Sankary
Oct 7, 2005·Journal of Toxicology and Environmental Health. Part a·Fu-Chuo PengRobert J Edwards
Sep 17, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Kohji TakaraTeruyoshi Yokoyama
Mar 9, 2004·The Journal of Pharmacology and Experimental Therapeutics·T MatsubaraY Yamazoe
Feb 28, 2003·The Journal of Pharmacology and Experimental Therapeutics·Sayeepriyadarshini AnakkHenry W Strobel
May 18, 1999·Toxicology and Applied Pharmacology·J M JacobsJ F Sinclair
Jul 23, 1999·Archives of Biochemistry and Biophysics·H ZhangB Yan
Jan 24, 1998·Biochemical and Biophysical Research Communications·G R RobertsonC Liddle
Apr 12, 2002·The Journal of Steroid Biochemistry and Molecular Biology·Robert Morfin
Aug 26, 1998·Chemico-biological Interactions·M MurrayA M Butler
Nov 25, 2000·Journal of Pharmacological and Toxicological Methods·M M CotreauD J Greenblatt
Jul 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·M J CheesmanP E Reilly
Aug 22, 2001·Biopharmaceutics & Drug Disposition·E HurhM G Lee
Oct 26, 2005·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·A HaduchW A Daniel
Sep 14, 2007·Journal of Pharmaceutical Sciences·Hiroshi Komura, Masahiro Iwaki
May 12, 2009·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Ansar A KhanGeny M M Groothuis

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