Apr 20, 2016

A MALDI-MS-based quantitative analytical method for endogenous estrone in human breast cancer cells

Scientific Reports
Kyoung-Jin KimYun-Gon Kim

Abstract

The level of endogenous estrone, one of the three major naturally occurring estrogens, has a significant correlation with the incidence of post-menopausal breast cancer. However, it is challenging to quantitatively monitor it owing to its low abundance. Here, we develop a robust and highly sensitive mass-assisted laser desorption/ionization mass spectrometry (MALDI-MS)-based quantitative platform to identify the absolute quantities of endogenous estrones in a variety of clinical specimens. The one-step modification of endogenous estrone provided good linearity (R(2) > 0.99) and significantly increased the sensitivity of the platform (limit of quantitation: 11 fmol). In addition, we could identify the absolute amount of endogenous estrones in cells of the breast cancer cell line MCF-7 (34 fmol/10(6) cells) by using a deuterated estrone as an internal standard. Finally, by applying the MALDI-MS-based quantitative method to endogenous estrones, we successfully monitored changes in the metabolic expression level of estrones (17.7 fmol/10(6) letrozole-treated cells) in MCF-7 cells resulting from treatment with an aromatase inhibitor. Taken together, these results suggest that this MALDI-MS-based quantitative approach may be a genera...Continue Reading

Mentioned in this Paper

Metabolic Process, Cellular
Surface Enhanced Laser Desorption Ionization Mass Spectrometry
Estrone Measurement
Pathogenic Organism
Aromatase Inhibitors
One-Step Dentin Bonding System
Menopause
Estrovarin
MCF-7 Cells
Estrogens, endocrine therapy drugs