Jul 3, 2007

A mammalian microRNA expression atlas based on small RNA library sequencing

Cell
Pablo LandgrafThomas Tuschl

Abstract

MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequence-complementary target mRNAs. In order to identify miRNAs and to assess their expression patterns, we sequenced over 250 small RNA libraries from 26 different organ systems and cell types of human and rodents that were enriched in neuronal as well as normal and malignant hematopoietic cells and tissues. We present expression profiles derived from clone count data and provide computational tools for their analysis. Unexpectedly, a relatively small set of miRNAs, many of which are ubiquitously expressed, account for most of the differences in miRNA profiles between cell lineages and tissues. This broad survey also provides detailed and accurate information about mature sequences, precursors, genome locations, maturation processes, inferred transcriptional units, and conservation patterns. We also propose a subclassification scheme for miRNAs for assisting future experimental and computational functional analyses.

  • References64
  • Citations1886

References

Mentioned in this Paper

Embryo
Northern Blotting
Biochemical Pathway
Conserved Sequence
Cortex Bone Disorders
Structure of Germinal Center of Lymph Node
Adrenal Cortex Diseases
Central Neuroblastoma
Colony-Forming Units, Hematopoietic
Genome

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