PMID: 11333018May 3, 2001Paper

A mammalian sequence-dependent upstream open reading frame mediates polyamine-regulated translation in yeast

RNA
Gregory J Mize, D R Morris

Abstract

In mammals, control of S-adenosylmethionine decarboxylase (AdoMetDC) translation is one component of a feedback network that regulates intracellular levels of the polyamines, spermidine, and spermine. AdoMetDC mRNA from mammals contains a highly conserved upstream open reading frame (uORF) within its leader sequence that confers polyamine-regulated suppression of translation on the associated downstream cistron. This regulation is mediated through an interaction that depends on the amino acid sequence of the uORF-encoded hexapeptide. It remains to be shown whether polyamines participate directly in this interaction or indirectly through a specialized signal transduction pathway. We show that Saccharomyces cerevisiae does not have a uORF associated with its AdoMetDC gene (SPE2) and that ribosome loading on the SPE2 mRNA is not positively influenced by polyamine depletion, as it is in mammalian cells. Nevertheless, the mammalian AdoMetDC uORF, when introduced into a polyamine auxotroph of yeast, conferred polyamine regulation of both translational efficiency and ribosome loading on the associated mRNA. This regulatory activity depended on the amino acid sequence encoded by the fourth and fifth codons of the uORF, as in mammalian ...Continue Reading

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Citations

Nov 26, 2013·Plant Science : an International Journal of Experimental Plant Biology·Albrecht G von ArnimJustin N Vaughn
Jul 15, 2006·Nucleosides, Nucleotides & Nucleic Acids·Kiyoshi OkadaAkio Kanai
May 1, 2009·Plant Physiology·Fatemeh RahmaniJohannes Hanson
Jun 11, 2004·Annual Review of Biochemistry·Jennifer A Doudna, Robert T Batey
Oct 29, 2013·Nucleic Acids Research·Klaus WethmarAchim Leutz
Dec 24, 2011·Trends in Parasitology·Erin Willert, Margaret A Phillips
Oct 17, 2018·The Journal of Biological Chemistry·Thomas E Dever, Ivaylo P Ivanov
Dec 14, 2001·The Journal of Biological Chemistry·Alexa RaneyDavid R Morris
Sep 2, 2020·Annual Review of Genetics·Thomas E DeverMatthew S Sachs

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