PMID: 7026705Oct 1, 1981Paper

A marker for megakaryocytes: serotonin accumulation in guinea pig megakaryocytes

The Journal of Laboratory and Clinical Medicine
P K Schick, M Weinstein

Abstract

Serotonin accumulation was studied in guinea pig megakaryocytes and platelets. Megakaryocyte suspensions, 80% to 90% pure, and platelet-rich plasma were used. 14C-serotonin was used to assay serotonin uptake. Maximum capacity for serotonin storage in 2.5 x 10(5) megakaryocytes was 4.2 nmol and in 2.5 x 10(8) platelets was 3.1 nmol. One thousand times as many platelets as megakaryocytes were used to normalize for protein content of the two cells. Thus the megakaryocyte's capacity for accumulating serotonin is equivalent to, if not greater than, that of the platelet. Serotonin accumulation in individual megakaryocytes and other hematopoietic cells was studied by autoradiography. Whole marrow suspensions were incubated with 3H-serotonin. Cells in whole marrow suspensions, cells in the pellet of the albumin density gradient, and purified megakaryocytes were then analyzed by autoradiography. The pellet of the albumin density gradient was inspected because it is known to contain megakaryocytes that are not recovered in the purified megakaryocyte suspensions. Examination of whole marrow cell suspensions revealed that virtually all megakaryocytes had taken up serotonin, but there was no evidence of serotonin accumulation in other hemat...Continue Reading

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