A mass spectrometric approach for the study of the metabolism of clomiphene, tamoxifen and toremifene by liquid chromatography time-of-flight spectroscopy

European Journal of Mass Spectrometry
Monica MazzarinoFrancesco Botrè

Abstract

In this paper, we discuss the capabilities of liquid chromatography coupled to mass spectrometry with a time-of flight system with accurate mass measurement for the detection and characterisation of drug metabolites in biological samples for anti-doping purpose. Urinary excretion samples of three selective oestrogen receptor modulators (SERMs) with a common triphenylethylene structure: clomiphene, toremifene, and tamoxifen, obtained after oral administration of a single dose of each drug, were analysed using a time-of-flight system, after automatic tuning and calibration of the equipment, in positive full scan mode using an electrospray ionisation source. Following this approach we detected most of all significant metabolites reported by others and postulated new metabolites, especially for toremifene, have been characterised: N-demethyl-3-hydroxy-4-methoxy-toremifene and 3- hydroxy-4-methoxy-toremifene; in addtiona to this, in the urinary excretion samples of toremifene some metabolites, without the characteristic chlorine isotope pattern, discarded in previous studies, that are also metabolites of tamoxifen, were identified. The lack of certified reference materials does not allow an accurate determination of the limit of det...Continue Reading

References

Feb 9, 2000·Investigational New Drugs·K Dhingra
Mar 25, 2000·Journal of the Society for Gynecologic Investigation·V C Jordan
Jul 31, 2001·Best Practice & Research. Clinical Obstetrics & Gynaecology·P Neven, I Vergote
Jan 25, 2002·Annals of the New York Academy of Sciences·L Plouffe, S Siddhanti
Jul 6, 2002·Critical Reviews in Oncology/hematology·Karla C MorelloMichael W DeGregorio
Nov 16, 2004·Cancer Treatment Reviews·John F R Robertson
Jul 5, 2007·Rapid Communications in Mass Spectrometry : RCM·Costas G GeorgakopoulosMichalis Koupparis
Apr 19, 2008·Rapid Communications in Mass Spectrometry : RCM·Michael S BeremanDavid C Muddiman

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Citations

Mar 12, 2011·Journal of Pharmaceutical and Biomedical Analysis·S F TeunissenJ H Beijnen
Oct 20, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gordon J Murray, Jonathan P Danaceau
Jan 23, 2015·Biomedical Chromatography : BMC·Juan C Domínguez-RomeroAntonio Molina-Díaz
Nov 6, 2018·Drug Testing and Analysis·Monica MazzarinoFrancesco Botrè
Nov 7, 2015·Biomedical Chromatography : BMC·Homayon Ahmad PanahiMonireh Chabouk
Mar 13, 2015·Pharmaceutical Research·Waseem HaririShaker A Mousa

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