A mass spectrometry plate reader: monitoring enzyme activity and inhibition with a Desorption/Ionization on Silicon (DIOS) platform

Chembiochem : a European Journal of Chemical Biology
Zhouxin ShenGary Siuzdak

Abstract

A surface-based laser desorption/ionization mass spectrometry assay that makes use of Desorption/Ionization on Silicon Mass Spectrometry (DIOS-MS) has been developed to monitor enzyme activity and enzyme inhibition. DIOS-MS has been used to characterize inhibitors from a library and then to monitor their activity against selected enzyme targets, including proteases, glycotransferase, and acetylcholinesterase. An automated DIOS-MS system was also used as a high-throughput screen for the activity of novel enzymes and enzyme inhibitors. On two different commercially available instruments, a sampling rate of up to 38 inhibitors per minute was accomplished, with thousands of inhibitors being monitored. The ease of applying mass spectrometry toward developing enzyme assays and the speed of surface-based assays such as DIOS for monitoring inhibitor effectiveness and enzyme activity makes it attractive for a broad range of screening applications.

References

Jan 13, 1998·Current Opinion in Biotechnology·J G Houston, M Banks
Jun 1, 1997·Current Opinion in Chemical Biology·J J Burbaum, N H Sigal
Jul 17, 1998·Current Opinion in Chemical Biology·G S SittampalamW P Janzen
Nov 18, 1998·Current Opinion in Chemical Biology·P B Fernandes
Nov 20, 1998·Journal of Mass Spectrometry : JMS·B N PramanikA K Ganguly
Jan 16, 1999·Current Opinion in Biotechnology·J E González, P A Negulescu
Jan 20, 1999·Cellular and Molecular Life Sciences : CMLS·C Traving, R Schauer
May 2, 2000·The Journal of Biological Chemistry·B BothnerG Siuzdak
Oct 18, 2000·Proceedings of the National Academy of Sciences of the United States of America·M T CancillaJ A Leary
Apr 3, 2001·Current Opinion in Chemical Biology·D Wahler, J L Reymond
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·J J ThomasG Siuzdak
Jun 26, 2001·Glycoconjugate Journal·R Schauer
Jan 5, 2002·Journal of Inherited Metabolic Disease·K D MacDermotA H Miners
May 23, 2002·Current Opinion in Chemical Biology·Catherine A Evans, Scott J Miller
Mar 26, 2003·Biochemistry·Matthías ThórólfssonAurora Martínez
Aug 16, 2003·Analytical Chemistry·Eden P GoGary Siuzdak

❮ Previous
Next ❯

Citations

Nov 12, 2005·Analytical and Bioanalytical Chemistry·Zhong GuoLin He
Jul 12, 2005·Analytical and Bioanalytical Chemistry·André Liesener, Uwe Karst
Aug 24, 2010·Analytical and Bioanalytical Chemistry·K P Law, James R Larkin
Apr 18, 2006·Journal of the American Society for Mass Spectrometry·Kenneth D GreisGregory F Davis
Mar 21, 2007·Journal of the American Society for Mass Spectrometry·Giuseppe GrassoKwon Joo Yeo
Jun 19, 2013·Analytical Chemistry·J Li, R H Lipson
Mar 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Trent R NorthenGary Siuzdak
Feb 1, 2006·Expert Review of Proteomics·Andrew M Dattelbaum, Srinivas Iyer
Aug 19, 2014·Current Opinion in Biotechnology·Tristan de RondTrent Northen
Oct 13, 2007·Current Opinion in Chemical Biology·D Allen AnnisCharles E Whitehurst
Sep 9, 2005·Chembiochem : a European Journal of Chemical Biology·Karin V NiessenKlaus T Wanner
Nov 10, 2006·Journal of Mass Spectrometry : JMS·Giuseppe GrassoKwon Joo Yeo
Feb 20, 2010·Mass Spectrometry Reviews·Jeroen J A van KampenTheo M Luider
Jan 22, 2011·Mass Spectrometry Reviews·Pawel L UrbanRenato Zenobi
Apr 10, 2013·Chembiochem : a European Journal of Chemical Biology·Sonia SernaNiels-Christian Reichardt
May 24, 2006·Carbohydrate Research·Kim LarsenKnud J Jensen
Nov 1, 2005·Journal of Pharmaceutical and Biomedical Analysis·Gejing Deng, Gautam Sanyal
Jun 23, 2005·Drug Discovery Today·Stanislav S RubakhinJonathan V Sweedler
Oct 27, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Youji ShimazakiAyaka Kimura
Jan 14, 2017·Journal of the American Chemical Society·Cunyu YanPerdita E Barran
Sep 13, 2006·Mass Spectrometry Reviews·Dominic S Peterson
Oct 14, 2005·Bioscience Reports·Ida Chiara Guerrera, Oliver Kleiner
Jun 7, 2011·ACS Combinatorial Science·Zachary A Gurard-LevinMilan Mrksich
Sep 3, 2020·Journal of the American Society for Mass Spectrometry·Yasuhide NaitoTakayuki Ohmura
Dec 17, 2016·Analytical Chemistry·Young-Lai ChoTae Geol Lee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.