A material decoy of biological media based on chitosan physical hydrogels: application to cartilage tissue engineering

Biochimie
Alexandra MontembaultAlain Domard

Abstract

The cartilage tissue has a limited self-regenerative capacity. Tissue-engineering represents a promising trend for cartilage repair. The present study was aimed to develop a biomaterial formulation by combining fragments of chitosan hydrogel with isolated rabbit or human chondrocytes. We first reported the properties of the constructs elaborated with rabbit chondrocytes and pure chitosan physical hydrogels with defined molecular weight, acetylation degree and polymer concentration. Morphological data showed that chondrocytes were not penetrating the hydrogels but tightly bound to the surface of the fragments and spontaneously formed aggregates of combined cell/chitosan. A significant amount of neo-formed cartilage-like extracellular matrix (ECM) was first accumulated in-between cells and hydrogel fragments and furthermore was widely distributed within the neo-construct. The optimal biological response was obtained with hydrogel fragments concentrated at 1.5% (w/w) of polymer made from a chitosan with a degree of acetylation between 30 and 40%. Such hydrogels were then mixed with human chondrocytes. The phenotype of the cells was analyzed by using chondrocytic (mRNA expression of mature type II collagen and aggrecan as well as s...Continue Reading

References

Nov 1, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M D BuschmannE B Hunziker
Oct 1, 1965·Histochemie. Histochemistry. Histochimie·J E Scott, J Dorling
Jun 1, 1984·Clinical Orthopaedics and Related Research·T KimuraK Ono
Nov 1, 1995·Plastic and Reconstructive Surgery·K T PaigeC A Vacanti
Aug 1, 1994·Journal of Biomedical Materials Research·L E FreedR Langer
Oct 6, 1994·The New England Journal of Medicine·M BrittbergL Peterson
Jan 1, 1996·Plastic and Reconstructive Surgery·K T PaigeC A Vacanti
Feb 1, 1997·Journal of Biomedical Materials Research·D A GrandeR Manji
Jul 1, 1997·Journal of Biomedical Materials Research·S NehrerM Spector
Apr 30, 1999·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·L A SolchagaA I Caplan
Jul 9, 1999·Medical & Biological Engineering & Computing·M Mattioli-BelmonteF Greco
Oct 8, 1999·Biomaterials·J X LuG Guillemin
Dec 22, 1999·Journal of Biomedical Materials Research·V F SechriestJ K Suh
Feb 16, 2000·Biomaterials·J S Temenoff, A G Mikos
Apr 25, 2000·Journal of Periodontology·Y M LeeC P Chung
Jul 8, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·M V RisbudR R Bhonde
Aug 22, 2000·Journal of Biomedical Materials Research·M S PonticielloF P Barry
Nov 15, 2000·Journal of Biomedical Materials Research·S PelletierE Dellacherie
Jan 12, 2001·Journal of Biomedical Materials Research·C JarryJ C Leroux
Dec 18, 2001·Journal of Biomedical Materials Research·Stephanie J Bryant, Kristi S Anseth
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·J KisidayA J Grodzinsky
Jan 25, 2003·Tissue Engineering·Dana L NettlesJerome A Gilbert
Apr 26, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Ranieri CanceddaRodolfo Quarto
May 14, 2003·Biomacromolecules·Christophe SchatzAlain Domard
Sep 9, 2004·Biomaterials·Alexandra MontembaultAlain Domard
Dec 4, 2004·Biomaterials·Alexandra MontembaultAlain Domard
Mar 15, 2005·Biomacromolecules·Alexandra MontembaultAlain Domard
Mar 23, 2005·Osteoarthritis and Cartilage·C D HoemannM D Buschmann

❮ Previous
Next ❯

Citations

Mar 5, 2009·Journal of Materials Science. Materials in Medicine·B HoffmannG Ziegler
Mar 23, 2011·Chemical Reviews·Biji Balakrishnan, R Banerjee
Dec 20, 2008·Journal de la Société de biologie·Marie-Thérèse CorvolFrançois Rannou
Mar 19, 2009·Tissue Engineering. Part a·Yi-Hsin ChenTai-Horng Young
Oct 26, 2011·Future Medicinal Chemistry·Jaydee Cabral, Stephen Carl Moratti
Jul 27, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Sébastien MalaiseLaurent David
Dec 11, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Lav Kumar SinghMeenakshi Singh
Dec 3, 2014·Carbohydrate Polymers·Aurélien BillardCatherine Ladavière
May 24, 2013·The Journal of Physical Chemistry. B·Rémi RouxThierry Delair
Mar 1, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hye-Rim LeeSun Hee Do
May 7, 2011·Biomaterials·S G LadetM-T M Corvol
Jun 15, 2007·Journal of Biomedical Materials Research. Part a·Darryl K KnightBrian G Amsden
Jun 15, 2007·Journal of Biomedical Materials Research. Part a·Gang ZhouJohn A Jansen
Sep 29, 2011·Journal of Tissue Engineering and Regenerative Medicine·M L Alves da SilvaN M Neves
Jun 2, 2009·Journal of Biomedical Materials Research. Part a·Guillaume R RagetlyDominique J Griffon
Sep 16, 2016·PloS One·Yi Wen ChaiJohn H Brekke
Sep 25, 2017·Journal of Biomedical Materials Research. Part a·Quentin DenostLaurence Bordenave
Mar 7, 2008·Nature·Sébastien LadetAlain Domard
Mar 25, 2019·Journal of Biomedical Materials Research. Part a·Yan SunXiao-Hong Li
Sep 15, 2020·Journal of Tissue Engineering·Hanxiang LeJianxun Ding
Oct 20, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Bingren TianJiayue Liu
Jan 29, 2020·International Journal of Biological Macromolecules·Maria Antonietta SabatinoClelia Dispenza
Nov 7, 2020·Progress in Biomaterials·A Ben BoualiS Féréol
Jan 7, 2017·Biotechnology Advances·Monika HospodiukIbrahim T Ozbolat
Nov 23, 2007·Biomacromolecules·Brian G AmsdenStephen N Shapka
Jul 4, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Fabrice BrunelThierry Delair
Mar 10, 2007·Biomacromolecules·N BoucardA Domard
Oct 12, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Nicolas SereniLaurent David
Oct 1, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Rocio Rivas-AraizaLaurent David
Nov 22, 2018·Biomacromolecules·Jimmy FaivreLaurent David
Apr 30, 2010·Colloids and Surfaces. B, Biointerfaces·Lyubov LukyanovaRichard Weinkamer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.