A maternal RNA localized in the yellow crescent is segregated to the larval muscle cells during ascidian development

Developmental Biology
B J Swalla, W R Jeffery

Abstract

A cDNA library prepared from one-cell zygotes of the ascidian Styela clava was screened with probes from isolated cellular fractions to identify clones encoding RNAs localized in the yellow crescent or myoplasm, a cytoskeletal domain with multiple developmental roles. The differential screen yielded five overlapping cDNA (Styela clava yellow crescent or ScYC) clones encoding a 1.2-kb polyadenylated RNA (yellow crescent or YC RNA) which is present throughout embryonic development. In situ hybridization confirmed that YC RNA is localized in the yellow crescent. Antisense probes containing the 3' region of YC RNA hybridize with multiple maternal and zygotic RNAs, suggesting sequence homologies with other transcripts. YC RNA was first detected during oogenesis when transcripts accumulate in the perinuclear region of vitellogenic oocytes and are gradually translocated to the cortex. The YC transcripts are localized in the cortex of unfertilized eggs but after fertilization segregate with the myoplasm to the yellow crescent. During cleavage most YC transcripts enter the primary muscle cell lineage. YC RNA is also present in the secondary muscle cells. The YC transcripts are retained in the myoplasm of oocytes and eggs extracted with ...Continue Reading

Citations

Jan 1, 1996·Developmental Genetics·B J Swalla, W R Jeffery
Sep 1, 1996·Somatic Cell and Molecular Genetics·C J Brown, S E Baldry
Oct 8, 2008·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Paulo P Amaral, John S Mattick
Aug 13, 1999·Development, Growth & Differentiation·T OkaH Nishida
Jan 11, 2000·Nucleic Acids Research·V A ErdmannJ Barciszewski
Oct 6, 1998·Annual Review of Biochemistry·A BashirullahH D Lipshitz
Nov 1, 2001·Annual Review of Cell and Developmental Biology·I M Palacios, D St Johnston
Jan 13, 2006·Developmental Biology·François ProdonChristian Sardet
Mar 17, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·François ProdonYasunori Sasakura

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