A mathematical model of iron import and trafficking in wild-type and Mrs3/4ΔΔ yeast cells

BMC Systems Biology
Joshua D Wofford, Paul A Lindahl

Abstract

Iron plays crucial roles in the metabolism of eukaryotic cells. Much iron is trafficked into mitochondria where it is used for iron-sulfur cluster assembly and heme biosynthesis. A yeast strain in which Mrs3/4, the high-affinity iron importers on the mitochondrial inner membrane, are deleted exhibits a slow-growth phenotype when grown under iron-deficient conditions. However, these cells grow at WT rates under iron-sufficient conditions. The object of this study was to develop a mathematical model that could explain this recovery on the molecular level. A multi-tiered strategy was used to solve an ordinary-differential-equations-based mathematical model of iron import, trafficking, and regulation in growing Saccharomyces cerevisiae cells. At the simplest level of modeling, all iron in the cell was presumed to be a single species and the cell was considered to be a single homogeneous volume. Optimized parameters associated with the rate of iron import and the rate of dilution due to cell growth were determined. At the next level of complexity, the cell was divided into three regions, including cytosol, mitochondria, and vacuoles, each of which was presumed to contain a single form of iron. Optimized parameters associated with im...Continue Reading

References

Apr 11, 1988·FEBS Letters·F RaguzziR R Crichton
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·P R GardnerC W White
Dec 23, 1999·The Journal of Biological Chemistry·J L Urbanowski, R C Piper
Apr 5, 2000·Biochemistry·S Ollagnier-De ChoudensM Fontecave
Jun 18, 2002·The Journal of Biological Chemistry·Ulrich MühlenhoffRoland Lill
Apr 2, 2003·Human Molecular Genetics·Emmanuel LesuisseAndrew Dancis
May 27, 2004·The Journal of Biological Chemistry·Liangtao Li, Jerry Kaplan
Sep 3, 2010·Genes & Development·William W ChenPeter K Sorger
Jan 8, 2011·Biochimica Et Biophysica Acta·K KrabM Wikström
May 17, 2011·Journal of Electron Microscopy·Masashi YamaguchiYoshimichi Kozuka
Jul 23, 2011·The Biochemical Journal·Heeyong YoonAndrew Dancis
Nov 4, 2011·Biochemistry·Allison L CockrellPaul A Lindahl
Jan 31, 2012·Journal of Theoretical Biology·J ChifmanR Laubenbacher
Aug 22, 2013·Current Opinion in Microbiology·Caryn E Outten, Angela-Nadia Albetel
Nov 19, 2013·PLoS Computational Biology·Simon Mitchell, Pedro Mendes
Mar 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Catherine B PoorChuan He
May 9, 2014·Biophysical Journal·Yee-Hung Mark Chan, Wallace F Marshall
Aug 8, 2014·Frontiers in Pharmacology·Caroline C Philpott, Moon-Suhn Ryu
Oct 16, 2014·Mitochondrion·Siddhesh ArasLawrence I Grossman
May 29, 2015·Biochemistry·Sean P McCormickPaul A Lindahl
Jun 24, 2015·European Journal of Cell Biology·Roland LillUlrich Mühlenhoff
Jun 28, 2015·European Journal of Cell Biology·Ulrich MühlenhoffRoland Lill
Aug 26, 2015·The Journal of Biological Chemistry·Joshua D Wofford, Paul A Lindahl
Feb 19, 2016·Journal of Proteome Research·Claudia AndreiniAntonio Rosato
Feb 7, 2017·PLoS Computational Biology·Julia ChifmanReinhard Laubenbacher

❮ Previous
Next ❯

Citations

Dec 20, 2020·Trends in Biotechnology·Alessandra Biz, Radhakrishnan Mahadevan

❮ Previous
Next ❯

Software Mentioned

R
ROS
vac
cyt
sim
cell
Mathematica
mit

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Expert Review of Hematology
Bibbin T PaulSuzy V Torti
Metal Ions in Life Sciences
Adrienne C Dlouhy, Caryn E Outten
Frontiers in Pharmacology
Caroline C Philpott, Moon-Suhn Ryu
© 2022 Meta ULC. All rights reserved