A matrix for DNA separation: genotyping and sequencing using poly(vinylpyrrolidone) solution in uncoated capillaries

Analytical Chemistry
Q Gao, E S Yeung

Abstract

We report a new sieving matrix for DNA separation based on commercially available poly(vinylpyrrolidone). The new sieving matrix has a very low viscosity at moderate concentrations, e.g., 27 cP at 4.5%. Its excellent self-coating property can reduce electroosmotic flow to a negligible level. Column regeneration between runs is very simple and effective. Successful separations were achieved in uncoated capillaries. For genotyping, we show that D1S80 and amelogenin sex determination system can be baseline separated as double-stranded DNA, and vWF, TH01, TPOX, and CSF1PO short tandem repeats can be separated with single-base resolution as single-stranded DNA in this new matrix. Sequencing of M13mp18 showed good resolution up to 500 bases in a solution of high-molecular-weight fraction extracted from commercially available PVP. The feasibility of adaptation to a multicapillary array system is discussed.

References

May 1, 1990·Analytical Chemistry·H DrossmanL M Smith
Jul 7, 1995·Journal of Chromatography. B, Biomedical Applications·H T Chang, E S Yeung

❮ Previous
Next ❯

Citations

May 19, 2001·Electrophoresis·J Drábek
Feb 24, 2001·Electrophoresis·M N Albarghouthi, A E Barron
Mar 22, 2001·Electrophoresis·Y MaE S Yeung
Mar 1, 2002·Electrophoresis·Marcella ChiariRoberto Consonni
Jun 3, 2006·Analytical and Bioanalytical Chemistry·Sheng-Bing YuJi-Ming Hu
Mar 28, 2001·Journal of Chromatography. a·D LiangB Chu
Aug 2, 2002·Journal of Chromatography. a·Wenwan Zhong, Edward S Yeung
Sep 7, 2002·Journal of Chromatography. a·Benjamin Chu, Dehai Liang
Sep 16, 1999·Journal of Chromatography. a·Z K Shihabi
Jan 8, 2000·Journal of Biochemical and Biophysical Methods·V Dolník
Sep 21, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·W Wei, E S Yeung
Jun 29, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·J Ren
Feb 5, 2003·Current Opinion in Biotechnology·Valessa Barbier, Jean Louis Viovy
Aug 14, 2010·Analytical Chemistry·Andrew Lee, George M Whitesides
May 8, 2004·Journal of Clinical Microbiology·Matthew M FerrisBabetta L Marrone
Nov 8, 2014·Biomicrofluidics·Lingling Wu, Stephen Levy
May 10, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jicun RenDan Wu
Dec 22, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Jackson Travis Del Bonis-O'DonnellDeborah K Fygenson
Mar 15, 2008·Biophysical Journal·Christian H RecciusH G Craighead

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.