A matrix-induced ion suppression method to normalize concentration in urinary metabolomics studies using flow injection analysis electrospray ionization mass spectrometry

Analytica Chimica Acta
Guan-Yuan ChenChing-Hua Kuo

Abstract

Normalizing the total urine concentration is important for minimizing bias in urinary metabolomics analysis comparisons. In this study, we report a matrix-induced ion suppression (MIIS)-based method to normalize concentration using flow injection analysis coupled with electrospray ionization mass spectrometry (FIA-ESI-MS). An ion suppression indicator (ISI) was spiked into urine samples, and the intensity of the extracted ion chromatogram (EIC) for ISI in a urine matrix was subtracted by the EIC for a blank solution and used to calculate the extent to which the signal was reduced by the urine matrix. A series dilution of pooled urine samples was used to correlate the urine concentration and level of ion suppression for ISI. A regression equation was used to estimate the relative concentration of unknown urine samples. The MIIS method was validated for linearity, precision and accuracy. We obtained a good correlation using a quadratic regression model for 1- to 32-fold urine dilutions (R(2)=0.998). The reproducibility (n=4) and intermediate precision (n=3) were below 5% RSD, and the accuracy ranged from 97.15% to 102.10%. The established method was used to estimate the relative concentrations of 16 urine samples, and the results...Continue Reading

References

Nov 10, 2000·Journal of the American Society for Mass Spectrometry·R KingT Olah
May 17, 2001·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·V ChadhaU S Alon
Jun 21, 2003·Clinical Chemistry·Thomas M Annesley
Sep 5, 2003·European Journal of Pharmacology·Yoshimi TsuchiyaKazuo T Suzuki
Oct 2, 2003·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Geeta G GyamlaniTimothy S Larson
Sep 27, 2005·Journal of Pharmaceutical and Biomedical Analysis·David L HeavnerMichael W Ogden
Jan 24, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Erin ChambersJeffrey R Mazzeo
Aug 21, 2007·Clinical Journal of the American Society of Nephrology : CJASN·Friedrich C Luft
Nov 15, 2008·Molecular & Cellular Proteomics : MCP·Kyoungmi KimRobert H Weiss
Jan 24, 2009·Journal of Analytical Toxicology·Edward J ConeDavid L Black
Feb 3, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Bethanne M WarrackDieter M Drexler
Mar 17, 2010·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Brandie D WagnerTheresa A Laguna
Jun 18, 2010·Kidney International·Sushrut S WaikarJoseph V Bonventre
Oct 19, 2010·Journal of Toxicology and Environmental Health. Part a·Kyu-Bong KimByung Mu Lee
Nov 10, 2010·Journal of Proteome Research·Joana CarrolaIola F Duarte
Dec 21, 2010·Analytica Chimica Acta·D RyanMegan Kendall
Apr 23, 2011·Bioanalysis·Renata BujakMichał Jan Markuszewski
Sep 21, 2011·Urologic Oncology·Sheila Ganti, Robert H Weiss
Dec 14, 2011·Journal of Proteome Research·Yu ChengWei Jia
Apr 12, 2012·Journal of the American Society of Nephrology : JASN·Chenjuan YaoA S Verkman
Jun 28, 2012·Biochimica Et Biophysica Acta·Thomas NilssonAnna Smedja Bäcklund
Sep 14, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Aihua ZhangXijun Wang
Oct 26, 2012·Omics : a Journal of Integrative Biology·Nele FriedrichHenri Wallaschofksi
Nov 9, 2012·American Journal of Physiology. Renal Physiology·Titilayo O IloriJanet D Klein
Jul 26, 2013·American Journal of Physiology. Renal Physiology·Nicholas G MossWilliam J Arendshorst
Oct 5, 2013·Biochimica Et Biophysica Acta·Rebecca E DayMatthew T Conner
Oct 11, 2013·American Journal of Physiology. Renal Physiology·Robert Moss, S Randall Thomas

❮ Previous
Next ❯

Citations

Jan 15, 2016·Journal of Chromatography. a·Yiman Wu, Liang Li
Feb 26, 2016·The Analyst·Marek Trojanowicz, Kamila Kołacińska
Apr 5, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marek Trojanowicz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.