A Matter of Timing: Contrasting Effects of Hydrogen Sulfide on Oxidative Stress Response in Shewanella oneidensis

Journal of Bacteriology
Genfu WuHaichun Gao

Abstract

Hydrogen sulfide (H2S), well known for its toxic properties, has recently become a research focus in bacteria, in part because it has been found to prevent oxidative stress caused by treatment with some antibiotics. H2S has the ability to scavenge reactive oxygen species (ROS), thus preventing oxidative stress, but it is also toxic, leading to conflicting reports of its effects in different organisms. Here, with Shewanella oneidensis as a model, we report that the effects of H2S on the response to oxidative stress are time dependent. When added simultaneously with H2O2, H2S promoted H2O2 toxicity by inactivating catalase, KatB, a heme-containing enzyme involved in H2O2 degradation. Such an inhibitory effect may apply to other heme-containing proteins, such as cytochrome cbb3 oxidase. When H2O2 was supplied 20 min or later after the addition of H2S, the oxidative-stress-responding regulator OxyR was activated, resulting in increased resistance to H2O2. The activation of OxyR was likely triggered by the influx of iron, a response to lowered intracellular iron due to the iron-sequestering property of H2S. Given that Shewanella bacteria thrive in redox-stratified environments that have abundant sulfur and iron species, our results ...Continue Reading

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Citations

Feb 15, 2017·Scientific Reports·Fen WanHaichun Gao
Mar 2, 2018·The Journal of Microbiology·Liu-Hui FuHua Zhang
Jan 24, 2016·Applied and Environmental Microbiology·Xi Jia Ooi, Kai Soo Tan
Feb 8, 2019·Journal of Drug Targeting·Lambert NzungizeJianping Xie
Mar 4, 2020·Microbial Biotechnology·Eleftheria NtagiaKorneel Rabaey
Aug 28, 2020·Frontiers in Microbiology·Say Yong NgWilfried Moreira
May 24, 2017·Proceedings of the National Academy of Sciences of the United States of America·Alexander MironovEvgeny Nudler
Jun 3, 2021·Antioxidants·Sofia S MendesLígia M Saraiva

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