PMID: 2496115May 1, 1989Paper

A maturation protein is essential for production of active forms of Lactococcus lactis SK11 serine proteinase located in or secreted from the cell envelope

Journal of Bacteriology
P VosW M de Vos

Abstract

The complete nucleotide sequence of a gene located immediately upstream of the Lactococcus lactis subsp. cremoris SK11 prtP gene encoding the cell envelope-attached proteinase was determined. This gene, designated prtM, was found to be transcribed from the same promotor region as was the proteinase gene but in the opposite direction. The prtM gene directed the expression in Escherichia coli of a protein with a size similar to the expected value of 33 kilodaltons, as deduced from the nucleotide sequence data. The derived amino acid sequence of the PrtM protein indicated the presence of a consensus lipoprotein signal sequence at the N terminus, which suggested that PrtM is a lipoprotein. Plasmids containing the prtM gene, the prtP gene, or both were constructed. Expression studies of L. lactis clones containing these plasmids showed that the prtM gene encodes a trans-acting activity involved in the maturation of cell envelope-located and -secreted forms of the SK11 proteinase.

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Citations

Sep 1, 1990·FEMS Microbiology Reviews·L L McKay, K A Baldwin
Sep 1, 1990·FEMS Microbiology Reviews·J Kok
May 1, 1993·The Journal of Dairy Research·P S TanW N Konings
Sep 1, 1993·FEMS Microbiology Reviews·G G Pritchard, T Coolbear
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Mar 24, 2012·PloS One·Nicolas TrémillonIsabelle Poquet
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Aug 24, 2004·Protein Science : a Publication of the Protein Society·Angelika KühleweinGerd Gemmecker
Dec 30, 2015·Molecular Microbiology·Cristina AlcántaraManuel Zúñiga
Feb 18, 2018·Applied and Environmental Microbiology·Simon van der ElsPeter A Bron
Feb 2, 2002·Applied and Environmental Microbiology·Henrike H Wemekamp-KamphuisTjakko Abee
Feb 20, 2007·Applied and Environmental Microbiology·Arno WegkampEddy J Smid
Apr 3, 2001·Applied and Environmental Microbiology·V EnoufG Corthier

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